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Effects of a pulsed light-induced stress on Enterococcus faecalis.
MedLine Citation:
PMID:  23035907     Owner:  NLM     Status:  Publisher    
AIMS: Pulsed Light (PL) technology is a surface decontamination process that can be used on food, packaging or water. PL efficiency may be limited by its low degree of penetration or because of a shadow effect. In these cases, surviving bacteria will be able to perceive PL as a stress. Such a stress was mimicked using low transmitted energy conditions, and its effects were investigated on the highly environmental adaptable bacterium Enterococcus faecalis V583. METHODS AND RESULTS: In these laboratory conditions, a complete decontamination of the artificially inoculated medium was performed using energy doses as low as 1.8 , while a treatment of 0.5, 1 and 1.2 led to a 2.2, 6 and 7-log10 cfu.mL(-1) reduction of the initial bacterial population, respectively. Application of a 0.5 pre-treatment allowed the bacteria to resist more efficiently a 1.2 subsequent PL dose. This 0.5 treatment increased the bacterial mutation frequency and affected the abundance of 19 proteins as revealed by a global proteome analysis. CONCLUSIONS: E. faecalis is able to adapt to a PL treatment, providing a molecular response to low-energy PL dose, leading to enhanced resistance to a subsequent treatment and increasing the mutation frequency. SIGNIFICANCE AND IMPACT OF THE STUDY: This study gives further insights on E. faecalis capacities to adapt and to resist to stress. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Sébastien Massier; Emeline Bouffartigues; Alain Rincé; Olivier Maillot; Marc G J Feuilloley; Nicole Orange; Sylvie Chevalier
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
LMSM, Laboratoire de Microbiologie-Signaux et Microenvironnement, EA 4312, Université de Rouen, France; Claranor, Avignon, France.
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