| Effects of pulse feeding of beet molasses on recombinant benzaldehyde lyase production by Escherichia coli BL21(DE3). | |
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MedLine Citation:
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PMID: 19547969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of fed-batch operation (FBO) strategy was investigated using pretreated-beet molasses, containing galactose that induces the lac promoter, on benzaldehyde lyase (BAL) production by recombinant Escherichia coli BL21(DE3)pLySs. After batch cultivation with 30 g l(-1) pretreated-beet molasses consisting of 7.5 g l(-1) glucose and 7.5 g l(-1) fructose, three FBO strategies were applied at dissolved oxygen (=40%) cascade to air-flow rate. In FBO1 when air-flow rate decreased considerably, feed was given to the system in pulses in such a way that pretreated-beet molasses concentration increased by 10 kg m(-3) (containing 2.5 g l(-1) glucose+2.5 g l(-1) fructose); however, decrease in air-flow rate demonstrated only the absence of glucose but not fructose. Thus, in FBO2 when fructose and glucose were completely utilized, pretreated-beet molasses was pulse-fed and its concentration increased by 10 g l(-1). In FBO3 with the decreased amount of pretreated-beet molasses (6 g l(-1)), shift response time from glucose to fructose consumption was avoided, and glucose and fructose consumptions were well correlated with air-flow rate, and the highest CX (8.04 g l(-1)) and BAL (2,315 U ml(-1)) production were obtained (t=24 h) with the highest substrate yield on cell and product formation. |
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Authors:
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Pinar Calik; Hande Levent |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-23 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 85 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-22 Completed Date: 2009-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 65-73 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Industrial Biotechnology and Metabolic Engineering Laboratory, Middle East Technical University, 06531, Ankara, Turkey. pcalik@metu.edu.tr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde-Lyases
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biosynthesis* Bioreactors / microbiology Culture Media / chemistry* Escherichia coli / metabolism* Escherichia coli Proteins / biosynthesis* Fermentation Molasses* Recombinant Proteins / biosynthesis Sweetening Agents / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Escherichia coli Proteins; 0/Recombinant Proteins; 0/Sweetening Agents; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.-/benzaldehyde lyase |
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