Document Detail

Effects of psoriatic scale extracts on oxidative metabolic responses in granulocytes assessed by chemiluminescence.
MedLine Citation:
PMID:  2921083     Owner:  NLM     Status:  MEDLINE    
The peptide fractions containing C5a des arg and anionic neutrophil activating peptide (ANAP) and the low-molecular-weight fractions containing LTB4 in psoriatic scale extracts exert a potent polymorphonuclear leukocyte (PMN) chemotactic activity. To evaluate whether any of these components in the psoriatic scale extracts stimulate a respiratory burst in PMNs, we studied luminol-dependent chemiluminescence (CL) in PMNs under their direct stimulation. After fractionation by molecular sieve chromatography, pooled peptide fractions eluting near the cytochrome c marker from eight samples induced high CL in PMNs, whereas those eluted in low-molecular-weight fractions after vitamin B12 marker were very weak in this response. Moreover, in six samples such low-molecular-weight fractions eluting near the vitamin B12 marker were found to have a suppressive effect on the oxidative metabolism of neutrophils induced by other stimulators. These findings suggest that the fraction of chemotactic peptides in psoriatic scales plays a major role in the activation of PMNs, inducing tissue damage in the psoriatic lesional epidermis. On the other hand, at least some low-molecular-weight components in psoriatic scales seem to exert an inhibitory effect on the respiratory burst induced by other activators.
T Kato; T Terui; H Takematsu; H Tagami
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammation     Volume:  13     ISSN:  0360-3997     ISO Abbreviation:  Inflammation     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-04-20     Completed Date:  1989-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600105     Medline TA:  Inflammation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  59-66     Citation Subset:  IM    
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
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MeSH Terms
Chemotactic Factors / isolation & purification*,  pharmacology
Epidermis / metabolism
Free Radicals
Granulocytes / drug effects,  metabolism*
Luminescent Measurements
Molecular Weight
Oxygen / metabolism
Psoriasis / metabolism*
Reg. No./Substance:
0/Chemotactic Factors; 0/Free Radicals; 7782-44-7/Oxygen

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