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Effects of protein supplementation frequency on physiological responses associated with reproduction in beef cows.
MedLine Citation:
PMID:  25412746     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objective of this experiment was to determine if frequency of protein supplementation impacts physiological responses associated with reproduction in beef cows. Fourteen non-pregnant, non-lactating beef cows were ranked by age and BW, and allocated to 3 groups. Groups were assigned to a 3 × 3 Latin square design, containing 3 periods of 21 d and the following treatments: 1) soybean meal ( SB: ) supplementation daily ( D: ), 2) SB supplementation 3 times/wk ( 3WK: ), and 3) SB supplementation once/wk ( 1WK: ). Within each period, cows were assigned to an estrus synchronization protocol; 100 μg of GnRH + controlled internal drug release ( CIDR: ) containing 1.38 g of progesterone ( P4: ) on d 1, 25 mg of PGF2α on d 8, and CIDR removal + 100 μg of GnRH on d 11. Grass-seed straw was offered for ad libitum consumption. Soybean meal was individually supplemented at a daily rate of 1 kg/cow (as-fed basis). Moreover, 3WK were supplemented on d 0, 2, 4, 7, 9, 11, 14, 16, and 18, whereas 1WK were supplemented on d 4, 11, and 18. Blood samples were collected from 0 (prior to) to 72 h after supplementation on d 11 and 18, and analyzed for plasma urea-N ( PUN: ). Samples collected from 0 to 12 h were also analyzed for plasma glucose, insulin, and P4 (d 18 only). Uterine flushing fluid was collected concurrently with blood sampling at 28 h for pH evaluation. Liver biopsies were performed concurrently with blood sampling at 0, 4, and 28 h, and analyzed for mRNA expression of carbamoylphosphate synthetase I ( CPS-I: ; h 28), and CYP2C19 and CYP3A4 (h 0 and 4 on d 18). Plasma urea-N concentrations were greater (P < 0.01) for 1WK vs. 3WK from 20 to 72 h, and greater (P < 0.01) for 1WK vs. D from 16 to 48 h and at 72 h after supplementation (treatment × hour interaction; P < 0.01). Moreover, PUN concentrations peaked at 28 h after supplementation for 3WK and 1WK (P < 0.01), and were greater (P < 0.01) at this time for 1WK vs. 3WK and D and for 3WK vs. D. Expression of CPS-I was greater (P < 0.01) for 1WK vs. D and 3WK. Uterine flushing pH tended (P ≤ 0.10) to be greater for 1WK vs. 3WK and D. No treatment effects were detected (P ≥ 0.15) on expression of CYP2C19 and CYP3A4, plasma glucose and P4 concentrations, whereas plasma insulin concentrations were greater (P ≤ 0.03) in D and 3WK vs. 1WK. Hence, decreasing frequency of protein supplementation did not reduce uterine flushing pH or plasma P4 concentrations, which are known to impact reproduction in beef cows.
Authors:
B I Cappellozza; R F Cooke; M M Reis; R S Marques; T A Guarnieri Filho; G A Perry; D B Jump; K A Lytle; D W Bohnert
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-20
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-21     Completed Date:  -     Revised Date:  2014-11-21    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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