Document Detail


Effects of protein extract from head-foot tissue of Oncomelania hupensis on the growth and gene expression of mother sporocysts of Schistosoma japonicum.
MedLine Citation:
PMID:  21800125     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Oncomelania hupensis is the intermediate host of Schistosoma japonicum. In the present study, we investigated the effects of protein extracts from head-foot or gland tissue of O. hupensis on mother sporocysts of S. japonicum cultured in vitro. In the presence of head-foot protein extract of snails from the native province Hunan, in-vitro-transformed mother sporocysts presented not only a longer survival time and stronger motility, but also a bigger size than parasites cultured with protein extracts of glands of the same snail or head-foot tissue of a non-native snail from the Hubei province. Using suppression subtractive hybridization, two subtractive libraries were constructed on the basis of RNA of sporocysts cultured with or without native snail head-foot protein extract. A number of 31 transcripts were found to be up-regulated. Sequence analyses revealed that they represented genes involved among others in metabolic process, electron transport chain, response to chemical stimulus, and oxidation-reduction processes. Opposite to that 20 down-regulated transcripts were among others related to pseudouridine synthesis, RNA processing, and ribosome biogenesis. The differential expression of three of these transcripts, encoding cytochrome c oxidase subunit 2 (Cox2), NADH-ubiquinone oxidoreductase (ND1), and dyskeratosis congenita 1 protein (DKC1), were confirmed by real-time PCR. The promoted development and the differential gene expression of cultured sporocysts under the influence of head-foot protein extract of native O. hupensis implied not only its ability to improve in vitro culture conditions for intramolluscan stages, it may also represent a priming result with respect to the identification and characterization of factors involved in the parasite-host interplay between S. japonicum and O. hupensis.
Authors:
Jun Yong Zhu; Qing Ye; Qin Ping Zhao; Zhen Ping Ming; Christoph G Grevelding; Ming Sen Jiang; Hui Fen Dong
Related Documents :
15369775 - Delivery of bcl-xl or its bh4 domain by protein transduction inhibits apoptosis in huma...
17057225 - A novel role for proline- and acid-rich basic region leucine zipper (par bzip) proteins...
8597045 - Bcl-2 family proteins: regulators of chemoresistance in cancer.
15342605 - Recombinant cyclophilins lack nuclease activity.
22950025 - Extracytoplasmic function σ factors of the widely distributed group ecf41 contain a fus...
18466225 - Suppression of a cold-sensitive mutation in ribosomal protein s5 reveals a role for rim...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-29
Journal Detail:
Title:  Parasitology research     Volume:  -     ISSN:  1432-1955     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Parasitology, School of Basic Medical Science, Wuhan University, Wuhan, Hubei Province, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Leishmania antimony resistance: what we know what we can learn from the field.
Next Document:  Fast counter-electroosmotic capillary electrophoresis-time-of-flight mass spectrometry of hyaluronan...