Document Detail


Effects of prostaglandin F(2α) analogues on endothelin-1-induced impairment of rabbit ocular blood flow: comparison among tafluprost, travoprost, and latanoprost.
MedLine Citation:
PMID:  20868682     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effects of prostaglandin F(2α) (PGF(2α)) analogues on the endothelin-1 (ET-1)-induced impairment of optic nerve head (ONH) blood flow and on ET-1-induced contraction in isolated ciliary artery segments. In male rabbits, one of four PGF(2α) analogues [0.0015% tafluprost, 0.0015% 15-hydroxyl tafluprost (15-OH tafluprost), 0.005% latanoprost, or 0.004% travoprost] was topically administered at various pretreatment times before intravitreal ET-1 injection. ONH blood flow was estimated by the laser speckle method, which expresses blood velocity as a quantitative index, the squared blur rate (SBR). SBR was measured just before (baseline value) and at 30, 60, and 120 min after ET-1 injection. SBR was significantly decreased from 4.47 ± 0.20 to 3.50 ± 0.10 (78.6 ± 2.4% of baseline) at 120 min after intravitreal ET-1 injection (5 pmol/eye). The ET-1-induced decrease was almost completely prevented by tafluprost and significantly inhibited by the other three analogues. The inhibitory effect lasted longest with tafluprost, as indicated by the effective pretreatment times (tafluprost: 90, 120, or 240 min; 15-OH tafluprost: 90, but not 120 or 240 min; latanoprost and travoprost: 120, but not 240 min). In vitro, the effects of PGF(2α) analogues on ET-1-induced contractions in male rabbit ciliary arteries were evaluated using an isometric tension recording system. Tafluprost, latanoprost, travoprost, and 15-OH tafluprost concentration-dependently relaxed the 10 nM ET-1-induced ciliary artery contraction. Improvement of the ocular circulation may be superior with tafluprost than with the other PGF(2α) analogues. The underlying mechanism may involve relaxation of ocular resistance vessels.
Authors:
Hiroaki Kurashima; Hiroshi Watabe; Noriko Sato; Sanae Abe; Naruhiro Ishida; Takeshi Yoshitomi
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2010-09-22
Journal Detail:
Title:  Experimental eye research     Volume:  91     ISSN:  1096-0007     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-29     Completed Date:  2010-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  853-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Research and Development Center, Santen Pharmaceutical Co. Ltd., Nara, Japan. hiroaki.kurashima@santen.co.jp
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Antihypertensive Agents / pharmacology*
Blood Flow Velocity / drug effects
Ciliary Arteries / physiology
Cloprostenol / analogs & derivatives*,  pharmacology
Dinoprost / analogs & derivatives*
Endothelin-1 / antagonists & inhibitors*,  toxicity
Isometric Contraction / drug effects
Laser-Doppler Flowmetry
Male
Muscle, Smooth, Vascular / physiology
Optic Disk / blood supply*
Prostaglandins F / pharmacology*
Prostaglandins F, Synthetic / pharmacology*
Rabbits
Regional Blood Flow / drug effects
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Endothelin-1; 0/Prostaglandins F; 0/Prostaglandins F, Synthetic; 0/tafluprost; 130209-82-4/latanoprost; 157283-68-6/travoprost; 40665-92-7/Cloprostenol; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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