Document Detail

Effects of propolis extract on sensitivity to chemotherapeutic agents in HeLa and resistant sublines.
MedLine Citation:
PMID:  17486684     Owner:  NLM     Status:  MEDLINE    
The effects of a propolis extract obtained by supercritical fluid extraction on sensitivity to chemotherapeutic agents were examined in HeLa cells and resistant sublines thereof. In addition, the actions of propolis and caffeic acid phenethyl ester (CAPE), a constituent of propolis, on the multidrug efflux transporter P-glycoprotein/MDR1, were evaluated in paclitaxel-resistant HeLa/TXL cells (MDR1-overexpressing cells). In HeLa cells, the sensitivity to paclitaxel and doxorubicin, substrates of MDR1, was unchanged in the presence of propolis. In HeLa/TXL cells, propolis increased sensitivity to these MDR1 substrates. The accumulation of Rhodamine123, also a substrate for MDR1, by HeLa/TXL cells increased in the presence of 50 microg/mL, but not 10 microg/mL, of the extract. However, the growth inhibition of HeLa/TXL cells by paclitaxel was not changed by CAPE, although the accumulation of Rhodamine123 increased significantly in the presence of 100 microm, but not 1 nM or 1 microm, CAPE. Collectively, the extract was suggested to inhibit the function of MDR1 and to increase the sensitivity to MDR1 substrates in HeLa/TXL cells, effects likely to be caused by constituents other than CAPE.
Kohji Takara; Megumi Fujita; Mika Matsubara; Tetsuya Minegaki; Noriaki Kitada; Noriaki Ohnishi; Teruyoshi Yokoyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  21     ISSN:  0951-418X     ISO Abbreviation:  Phytother Res     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-27     Completed Date:  2007-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  841-6     Citation Subset:  IM    
Department of Hospital Pharmacy, Faculty of Pharmaceutical Sciences, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / administration & dosage,  pharmacology*,  therapeutic use
Cell Proliferation / drug effects*
Cisplatin / pharmacology
Drug Resistance, Multiple / drug effects
Drug Resistance, Neoplasm / drug effects
Fluorouracil / pharmacology
Hela Cells / drug effects
Inhibitory Concentration 50
Paclitaxel / pharmacology
Propolis / administration & dosage,  pharmacology*,  therapeutic use
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 15663-27-1/Cisplatin; 33069-62-4/Paclitaxel; 51-21-8/Fluorouracil; 9009-62-5/Propolis

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