| Effects of progesterone and anti-progestin (mifepristone) treatment on proliferation and apoptosis of the human ovarian cancer cell line, OVCAR-3. | |
| | |
MedLine Citation:
|
PMID: 16525653 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The study examined the effects of various progesterone and mifepristone concentrations on the proliferation and apoptosis of the human ovarian cancer cell line, OVCAR-3. OVCAR-3 cells were incubated with progesterone and mifepristone at concentrations ranging from 10(-3) to 10(-9) M. Proliferation and apoptosis were studied by means of inverted optical microscopy, DAPI staining, and crystal violet assay. Immunoblotting was used to study the regulation of the apoptosis-related proteins, bcl-2, caspase-3 and PARP, after incubation with various reagents. OVCAR-3 cell density was increased by progesterone concentrations of 10(-5) M or less, and decreased by 10(-3) M progesterone. DAPI staining showed no apoptotic bodies. Mifepristone concentrations of 10(-3) and 10(-4) M reduced the OVCAR-3 cell density. Immunoblotting showed PARP cleavage in the presence of mifepristone 10(-4) M. Caspase-3 and bcl-2 expression was reduced by mifepristone 10(-4) and 10(-7) M. These results suggest that progesterone has a paradoxical effect on OVCAR-3 cell proliferation, stimulating it at low concentrations and inhibiting it at high concentrations, potentially through a caspase-independent non-apoptotic death pathway. Mifepristone seems to inhibit OVCAR-3 cell proliferation by down-regulating bcl-2 and up-regulating caspase-3 activity. These preliminary results suggest that progesterone and mifepristone have beneficial effects in ovarian cancer. |
| | |
Authors:
|
Raffaèle Fauvet; Charlotte Dufournet Etienne; Christophe Poncelet; Annie-France Bringuier; Gérard Feldmann; Emile Daraï |
Related Documents
:
|
12504573 - Apoptosis modulation of epstein-barr virus-encoded latent membrane protein 1 in the epi... 15309463 - Interaction of polyunsaturated fatty acids and sodium butyrate during apoptosis in ht-2... 19810103 - Resveratrol enhances p53 acetylation and apoptosis in prostate cancer by inhibiting mta... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Oncology reports Volume: 15 ISSN: 1021-335X ISO Abbreviation: Oncol. Rep. Publication Date: 2006 Apr |
Date Detail:
|
Created Date: 2006-03-09 Completed Date: 2006-07-27 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: Greece |
Other Details:
|
Languages: eng Pagination: 743-8 Citation Subset: IM |
Affiliation:
|
Service de Gynécologie-Obstétrique, Hôpital Tenon, Faculté Saint-Antoine, Assistance Publique des Hôpitaux de Paris, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Caspase 3 Caspases / metabolism Cell Line, Tumor Cell Proliferation / drug effects* Dose-Response Relationship, Drug Female Gentian Violet Hormone Antagonists / pharmacology Humans Immunoblotting Indoles Microscopy, Fluorescence Mifepristone / pharmacology* Ovarian Neoplasms / metabolism, pathology Poly(ADP-ribose) Polymerases / metabolism Progesterone / pharmacology* Proto-Oncogene Proteins c-bcl-2 / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Hormone Antagonists; 0/Indoles; 0/Proto-Oncogene Proteins c-bcl-2; 47165-04-8/DAPI; 548-62-9/Gentian Violet; 57-83-0/Progesterone; 84371-65-3/Mifepristone; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The antitumor effect of a novel differentiation inducer, 2, 2-Bis (4-(4-amino-3-hydroxyphenoxy) phen...
Next Document: Alteration of hTERT full-length variant expression level showed different gene expression profiles a...