| Effects of probiotics on methionine choline deficient diet-induced steatohepatitis in rats. | |
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MedLine Citation:
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PMID: 22706738 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background/aims: Intestinal bacteria induce endogenous signals that play a pathogenic role in hepatic insulin resistance and non-alcoholic fatty liver disease. Probiotics could modulate the gut flora and could influence the gut-liver axis. We aimed to investigate the preventive effect of two probiotic mixtures on the methionine choline-deficient diet-induced non-alcoholic steatohepatitis model in rats. Methods: Two studies, short- term (2 weeks) and long-term (6 weeks), were carried out using 60 male Wistar rats. The 2-week study included six groups. Rats were fed with methionine choline-deficient diet or pair-fed control diet and were given a placebo or one of two probiotic mixtures (Pro-1 and Pro-2) by orogas- tric gavage. In the 6-week study, rats were allocated into four groups and were fed with methionine choline-deficient diet or pair-fed control di- et and given a placebo or Pro-2. At the end of the 2- and 6-week periods, blood samples were obtained, the animals were sacrificed, and liver tis- sues were removed. Serum alanine aminotransferase activity was determined; histologic and immunohistochemical analysis was performed for steatosis, inflammation, protein expression of tumor necrosis factor-α, and apoptosis markers. Results: In both studies, methionine choline-de- ficient diet caused an elevation of serum alanine aminotransferase activity, which was slightly reduced by Pro-1 and Pro-2. In the 2- and 6-we- ek studies, feeding with methionine choline-deficient diet resulted in steatosis and inflammation, but not fibrosis, in all rats. In the 2-week study, in rats fed with methionine choline-deficient diet and given Pro-1, steatosis and inflammation were present in 2 of 6 rats. In rats fed with met- hionine choline-deficient diet and given Pro-2, steatosis was detected in 3 of 6 rats, while inflammation was present in 2 of 6 rats. In the 6-we- ek study, in rats fed with methionine choline-deficient diet and given Pro-2, steatosis and inflammation were present in 3 of 6 rat livers. In both the 2- and 6-week studies, methionine choline-deficient diet resulted in tumor necrosis factor-α, proapoptotic Bax, caspase 3, caspase 8, and an- ti-apoptotic Bcl-2 expression in all rat livers. Pro-1 and Pro-2 treatment influenced protein expression involved in apoptosis and tumor necrosis factor-α in varying degrees. Conclusions: Pro-1 and Pro-2 decrease methionine choline-deficient diet-induced steatohepatitis in rats. The pre- ventive effect of probiotics may be due, in part, to modulation of apoptosis and their anti-inflammatory activity. |
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Authors:
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Nermin Karahan; Mehmet Işler; Ahmet Koyu; Aynur G Karahan; Gülden Başyığıt Kiliç; Ibrahim Metin Cırış; Recep Sütçü; Ibrahim Onaran; Hakan Cam; Muharrem Keskın |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology Volume: 23 ISSN: 1300-4948 ISO Abbreviation: Turk J Gastroenterol Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-06-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9515841 Medline TA: Turk J Gastroenterol Country: Turkey |
Other Details:
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Languages: eng Pagination: 110-21 Citation Subset: IM |
Affiliation:
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Süleyman Demirel University Faculty of Medicine, Department of Pathology, Isparta, Turkey. nerminkarahan@hotmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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