Document Detail


Effects of preparation method, age, and plating technique of thin agar layer media on recovery of Escherichia coli O157:H7 injured by sodium chloride.
MedLine Citation:
PMID:  11576689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The thin agar layer (TAL) method was experimentally tested to determine its ability to recover Escherichia coli O157:H7 injured by sodium chloride (NaCl). Cells grown in Brain Heart Infusion broth with 0%, 5%, or 7.5% (w/v) NaCl were spread and spiral plated onto Tryptic Soy agar (TSA), MacConkey Sorbitol agar (MSA), and TSA/MSA TAL combinations. Generally, TSA recovered more injured cells than TAL (p < or =0.05), and TAL recovered more cells than MSA (p < or =0.05). Preparation mode (two vs. three layers) and age (0, 1, or 7 days) of TAL had negligible effect on resuscitation of injured cells (p > 0.05). TAL, which is conventionally used to recover heat, cold, and acid-injured foodborne pathogens, may be used to recover NaCl-injured E. coli O157:H7.
Authors:
M N Hajmeer; D Y Fung; J L Marsden; G A Milliken
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microbiological methods     Volume:  47     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-09-28     Completed Date:  2002-04-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  249-53     Citation Subset:  IM    
Affiliation:
Department of Population Health and Reproduction, University of California-Davis, Davis, CA 95616, USA. mnhajmeer@ucdavis.edu
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MeSH Terms
Descriptor/Qualifier:
Acids
Agar*
Bacteriological Techniques / methods*
Cell Culture Techniques / methods*
Colony Count, Microbial
Culture Media / chemistry
Escherichia coli O157 / cytology,  drug effects*,  growth & development,  physiology*
Sensitivity and Specificity
Sodium Chloride / pharmacology*
Chemical
Reg. No./Substance:
0/Acids; 0/Culture Media; 7647-14-5/Sodium Chloride; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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