| Effects of posture on regional pulmonary blood flow in rats as measured by PET. | |
| | |
MedLine Citation:
|
PMID: 19926822 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Using small animal PET with (68)Ga-radiolabeled human albumin microspheres (Ga-68-microspheres), we investigated the effect of posture on regional pulmonary blood flow (PBF) in normal rats. This in vivo method is noninvasive and quantitative, and it allows for repeated longitudinal measurements. The purpose of the experiment was to quantify spatial differences in PBF in small animals in different postures. Two studies were performed in anesthetized, spontaneously breathing Wistar rats. Study 1 was designed to determine PBF in the prone and supine positions. Ga-68-microspheres were given to five prone and eight supine animals. We found that PBF increased in dorsal regions of supine animals (0.75) more than in prone animals (0.70; P = 0.037), according to a steeper vertical gradient of flow in supine than in prone animals. No differences in spatial heterogeneity were detected. Study 2 was designed to determine the effects of tissue distribution on PBF measurements. Because microspheres remained fixed in the lung, PET was performed on animals in the position in which they received Ga-68-microsphere injections and thereafter in the opposite posture. The distribution of PBF showed a preference for dorsal regions in both positions, but the distribution was dependent on the position during administration of the microspheres. We conclude that PET using Ga-68-microspheres can detect and quantify regional PBF in animals as small as the rat. PBF distributions differed between the prone and supine postures and were influenced by the distribution of lung tissue within the thorax. |
| | |
Authors:
|
Torsten Richter; Ralf Bergmann; Jens Pietzsch; Inge K?zle; Frank Hofheinz; Eik Schiller; Maximilian Ragaller; J?rg van den Hoff |
Publication Detail:
|
Type: Journal Article Date: 2009-11-19 |
Journal Detail:
|
Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 108 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-02-01 Completed Date: 2010-04-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 422-9 Citation Subset: IM |
Affiliation:
|
Department of Anesthesia and Critical Care, University Hospital Carl Gustav Carus, Technische Universit?t Dresden, Dresden, Germany. torsten.richter@uniklinikum-dresden.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Capillaries / drug effects Gallium Radioisotopes / administration & dosage, diagnostic use, pharmacokinetics Image Processing, Computer-Assisted Lung / physiology*, radionuclide imaging* Male Microspheres Positron-Emission Tomography Posture / physiology* Prone Position / physiology Pulmonary Circulation / physiology* Rats Rats, Wistar Software Supine Position / physiology Thorax / physiology, radionuclide imaging Tissue Distribution Tomography, Emission-Computed, Single-Photon |
| Chemical | |
Reg. No./Substance:
|
0/Gallium Radioisotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Liver apoptosis is age dependent and is reduced by activation of peroxisome proliferator-activated r...
Next Document: Obesity, adipokines, and lung disease.