Document Detail


Effects of polyunsaturated fatty acids on cardiac voltage-activated K(+) currents in adult ferret cardiomyocytes .
MedLine Citation:
PMID:  12195273     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
This study was carried out in adult ferret cardiomyocytes to investigate the effects of the n-3 polyunsaturated fatty acids (PUFAs) on voltage-gated K(+) currents. We report that the two outward K(+) currents: the transient outward K(+) current (I(to)) and the delayed rectifier K(+) current (I(K)), are both inhibited by the n-3 PUFAs, while the inwardly rectifying K(+) current (I(K1)) is unaffected by the n-3 PUFAs. Docosahexaenoic acid (C22:6n-3, DHA) produced a concentration dependent suppression of I(to) and I(K) in adult ferret cardiomyocytes with an IC(50) of 7.5 and 20 micromol/L, respectively; but not I(K1). In addition, eicosapentaenoic acid (C20:5n-3, EPA) had the effects on the three K(+) channels similar to DHA. Arachidonic acid (C20:4n-6, AA) at 5 or 10 micromol/L, after an initial inhibitory effect on I(K), caused an activation of I(K),AA which was prevented by pretreatment with indomethacin, a cyclooxygenase inhibitor. Monounsaturated and saturated fatty acids, which are not antiarrhythmic, lack the effects on these K(+) currents. Our results demonstrate that the n-3 PUFAs inhibit cardiac I(to) and I(K) with much less potency compared to their effects on cardiac Na(+) and Ca(2+) currents as we reported previously. This inhibition of the cardiac ion currents by the n-3 PUFAs may contribute to their antiarrhythmic actions.
Authors:
Yong-Fu Xiao; James P Morgan; Alexander Leaf
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Sheng li xue bao : [Acta physiologica Sinica]     Volume:  54     ISSN:  0371-0874     ISO Abbreviation:  Sheng Li Xue Bao     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730130R     Medline TA:  Sheng Li Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  271-81     Citation Subset:  IM    
Affiliation:
Stem Cell Research Laboratory, Beth Israel Deaconess Medical Center; Massachusetts General Hospital; Department of Medicine, Harvard Medical School, Boston, MA 02215, USA. yxiao@caregroup.harvard.edu
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
DA12762/DA/NIDA NIH HHS; DK38165/DK/NIDDK NIH HHS; HL62284/HL/NHLBI NIH HHS

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