| Effects of polycyclic aromatic hydrocarbon-containing oil mixtures on generation of reactive oxygen species and cell viability in MCF-7 breast cancer cells. | |
| | |
MedLine Citation:
|
PMID: 17558805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Clarified slurry oil (CSO), and two crude oil samples, Belridge heavy crude oil (BHCO) and Lost Hills light crude oil (LHLCO), were examined for their ability to generate reactive oxygen species (ROS) in MCF-7 cells. Intracellular ROS and cell viability were determined in a flow cytometer using dihydroxyrhodamine 123 and propidium iodide, respectively. In experiments with short-term exposure, single-cell suspensions were loaded with the fluorescent probes and then treated with the oil samples (1 or 10 ppm). Measurements were made at 5, 15, 30, 60, and 90 min after addition of oil samples. In experiments with longer term exposure, preconfluent cell cultures were treated with oil samples for 6, 12, or 24 h prior to preparing single-cell suspensions. Both short-term and longer term treatment with oil samples resulted in elevated generation of reactive oxygen species (ROS). Cell cultures also were treated with benzo[a]pyrene, a polycyclic aromatic hydrocarbon detected in all three oil samples. Treatment with benzo[a]pyrene produced a significant increase in levels of ROS. The present findings suggest that oil samples with higher concentrations of polycyclic aromatic hydrocarbons may exert adverse effects on human mammary epithelial tissue through induction of oxidative stress. |
| | |
Authors:
|
Bayram Yilmaz; John Ssempebwa; Carl R Mackerer; Kathleen F Arcaro; David O Carpenter |
Related Documents
:
|
15804435 - Newborn neurons acquire high levels of reactive oxygen species and increased mitochondr... 19285105 - Regulation of cell proliferation by nadph oxidase-mediated signaling: potential roles i... 1617505 - Measurement of bone cell metabolism in vitro using mass spectrometry. 8356915 - Studies on the mechanism of the cytotoxic action of the mitomycin antibiotics in hypoxi... 3088215 - Modulation in vitro of immune parameters in homosexual males with the preclinical compl... 16627975 - Chemosensitizing multiple drug resistance of human carcinoma by bicyclol involves atten... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of toxicology and environmental health. Part A Volume: 70 ISSN: 1528-7394 ISO Abbreviation: J. Toxicol. Environ. Health Part A Publication Date: 2007 Jul |
Date Detail:
|
Created Date: 2007-06-11 Completed Date: 2007-06-20 Revised Date: 2007-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 100960995 Medline TA: J Toxicol Environ Health A Country: England |
Other Details:
|
Languages: eng Pagination: 1108-15 Citation Subset: IM |
Affiliation:
|
Faculty of Medicine, Department of Physiology, Yeditepe University, Istanbul, Turkey. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Breast
/
drug effects Breast Neoplasms / pathology* Female Humans Oxidative Stress Petroleum / toxicity* Polycyclic Hydrocarbons, Aromatic / toxicity* Reactive Oxygen Species / metabolism* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
5D43TW00636/TW/FIC NIH HHS; R01 ES049795-01A1/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Petroleum; 0/Polycyclic Hydrocarbons, Aromatic; 0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Retrospective exposure assessment of airborne asbestos related to skilled craftsmen at a petroleum r...
Next Document: A comparison of the pulmonary inflammatory potential of different components of yeast cell wall.