| Effects of platelet-derived growth factor-BB on the metabolic function and morphologic features of equine tendon in explant culture. | |
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MedLine Citation:
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PMID: 16948608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the effects of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on the metabolic function and morphologic features of equine superficial digital flexor tendon (SDFT) in explant culture. Animals-6 euthanized horses (2 to 5 years old). METHODS: Forelimb SDFT explants were cultured for 6 days as untreated control specimens or treated with rhPDGF-BB (1, 10, 50, or 100 ng/mL of medium). Treatment effects on explant gene expression were evaluated via real-time PCR analysis of collagen type I, collagen type III, PDGF-A, and PDGF-B mRNA. Explants were assayed for total collagen, glycosaminoglycan, and DNA content; histologic changes were assessed via H and E staining and immunohistochemical localization of collagen types I and III. RESULTS: No morphologic or proliferative changes were detected in tendon explant sections. After high-dose rhPDGF-BB treatment, gene expression of collagen types I and III was increased and decreased, respectively. Expression of PDGF-A and PDGF-B mRNA was significantly increased at 24 hours, but later decreased to have few or negative autoinductive effects. Although PDGF gene expression waned after 48 hours of culture, collagen type I gene expression was significantly increased at 48 hours and reached peak value on day 6. Glycosaminoglycan and DNA content of explants were unchanged with rhPDGF-BB treatment. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that rhPDGF-BB use may be of benefit in the repair of equine tendon, particularly through induction of collagen type I mRNA. Positive autoinductive effects of PDGF-BB in equine SDFT explants were detected early following culture medium supplementation, but these diminished with time. |
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Authors:
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Jennifer L Haupt; Brandon P Donnelly; Alan J Nixon |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of veterinary research Volume: 67 ISSN: 0002-9645 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-04 Completed Date: 2006-10-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: United States |
Other Details:
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Languages: eng Pagination: 1595-600 Citation Subset: IM |
Affiliation:
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Comparative Orthopaedics Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Collagen Type I / genetics, metabolism Collagen Type III / genetics, metabolism DNA / genetics Dose-Response Relationship, Drug Gene Expression / drug effects Glycosaminoglycans / metabolism Horses / metabolism* Immunohistochemistry / veterinary Platelet-Derived Growth Factor / genetics, metabolism, pharmacology* RNA, Messenger / biosynthesis, genetics Reverse Transcriptase Polymerase Chain Reaction / veterinary Tendons / drug effects*, metabolism* Tissue Culture Techniques |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/Collagen Type III; 0/Glycosaminoglycans; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/platelet-derived growth factor A; 0/platelet-derived growth factor BB; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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