Document Detail

Effects of platelet-derived growth factor-BB on the metabolic function and morphologic features of equine tendon in explant culture.
MedLine Citation:
PMID:  16948608     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the effects of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on the metabolic function and morphologic features of equine superficial digital flexor tendon (SDFT) in explant culture. Animals-6 euthanized horses (2 to 5 years old). METHODS: Forelimb SDFT explants were cultured for 6 days as untreated control specimens or treated with rhPDGF-BB (1, 10, 50, or 100 ng/mL of medium). Treatment effects on explant gene expression were evaluated via real-time PCR analysis of collagen type I, collagen type III, PDGF-A, and PDGF-B mRNA. Explants were assayed for total collagen, glycosaminoglycan, and DNA content; histologic changes were assessed via H and E staining and immunohistochemical localization of collagen types I and III. RESULTS: No morphologic or proliferative changes were detected in tendon explant sections. After high-dose rhPDGF-BB treatment, gene expression of collagen types I and III was increased and decreased, respectively. Expression of PDGF-A and PDGF-B mRNA was significantly increased at 24 hours, but later decreased to have few or negative autoinductive effects. Although PDGF gene expression waned after 48 hours of culture, collagen type I gene expression was significantly increased at 48 hours and reached peak value on day 6. Glycosaminoglycan and DNA content of explants were unchanged with rhPDGF-BB treatment. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that rhPDGF-BB use may be of benefit in the repair of equine tendon, particularly through induction of collagen type I mRNA. Positive autoinductive effects of PDGF-BB in equine SDFT explants were detected early following culture medium supplementation, but these diminished with time.
Jennifer L Haupt; Brandon P Donnelly; Alan J Nixon
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  67     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1595-600     Citation Subset:  IM    
Comparative Orthopaedics Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Collagen Type I / genetics,  metabolism
Collagen Type III / genetics,  metabolism
DNA / genetics
Dose-Response Relationship, Drug
Gene Expression / drug effects
Glycosaminoglycans / metabolism
Horses / metabolism*
Immunohistochemistry / veterinary
Platelet-Derived Growth Factor / genetics,  metabolism,  pharmacology*
RNA, Messenger / biosynthesis,  genetics
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Tendons / drug effects*,  metabolism*
Tissue Culture Techniques
Reg. No./Substance:
0/Collagen Type I; 0/Collagen Type III; 0/Glycosaminoglycans; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/platelet-derived growth factor A; 0/platelet-derived growth factor BB; 9007-49-2/DNA

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