Document Detail

Effects of the pig renal epithelial cell line LLC-PK1 and its conditioned medium on the phenotype of porcine microglia in vitro.
MedLine Citation:
PMID:  17337088     Owner:  NLM     Status:  MEDLINE    
Microglia are dispersed throughout the central nervous system. Under physiological circumstances they display a 'ramified' resting phenotype. In different neuropathologies microglia reversibly transform into the activated form, an amoeboid phagocyte with a broad spectrum of immune effector functions. In this study, a coculture of porcine microglia and the pig renal epithelial cell line LLC-PK1 was used to investigate microglial cell biology. The morphology of the cocultures was elucidated as well as the functionality of the microglia cells by proliferation, superoxide and phagocytosis assays. Our results demonstrate that direct intercellular contact between the two cell types was necessary for microglia to acquire a ramified morphology. Moreover, the conditioned medium of the renal cells promoted proliferation of microglia, inhibited giant cell formation and stimulated microglia to retain their capability to generate superoxide and to perform phagocytosis. In conclusion, we have constructed a cell culture system showing differentiation of microglia in vitro and keeping them in optimal conditions.
Bart R Tambuyzer; Ivo Lambrichts; Marc Lenjou; Etienne J Nouwen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-06
Journal Detail:
Title:  European journal of cell biology     Volume:  86     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-20     Completed Date:  2007-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  221-32     Citation Subset:  IM    
Laboratory of Neurobiology and Neuropharmacology, Department of Biomedical Sciences, University of Antwerp (UA), Universiteitsplein 1, B-2610 Wilrijk, Belgium.
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MeSH Terms
Cell Communication*
Cell Differentiation
Cell Proliferation
Cell Shape
Coculture Techniques
Culture Media, Conditioned / metabolism
Epithelial Cells / cytology,  metabolism,  physiology*,  ultrastructure
Giant Cells / cytology
Kidney / cytology,  physiology
LLC-PK1 Cells
Microglia / cytology,  metabolism,  physiology*,  ultrastructure
Microscopy, Confocal
Microscopy, Electron, Transmission
Superoxides / metabolism
Time Factors
Reg. No./Substance:
0/Culture Media, Conditioned; 11062-77-4/Superoxides

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