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Effects of physical exercise on butyrylcholinesterase in obese adolescents.
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PMID:  23271933     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to evaluate the effect of a 12 week program of physical exercise (PE) on butyrylcholinesterase (BChE) in obese adolescents. This study compared obese adolescents (N = 54) before and after PE, regarding the relative intensity (RI) and activity of different molecular forms (G1, G2, G4 and G1-ALB) of BChE found in plasma. Waist circumference (WC) and lipid profile were also assessed before and after PE. It was shown that before PE, mean plasma BChE activity was significantly higher in obese than in non-obese adolescents and that it was significantly reduced after PE, becoming similar to that found in non-obese adolescents. Lipid profile and WC also changed in response to PE. These results are consistent with studies that found a correlation between BChE and lipid metabolism and suggest that PE may have led to a physiological regularization of plasma BChE activity. Although mean BChE activity of each isoform was significantly reduced by PE, their RI did not change. This is in accordance with a previous suggestion that this proportion is maintained under factors such as obesity, and may therefore be important for BChE functions.
Authors:
Isabela M W Silva; Neiva Leite; Dellyana Boberg; Thais J Chaves; Gerusa M Eisfeld; Gisele M Eisfeld; Gleyse F Bono; Ricardo L R Souza; Lupe Furtado-Alle
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Publication Detail:
Type:  Journal Article     Date:  2012-10-09
Journal Detail:
Title:  Genetics and molecular biology     Volume:  35     ISSN:  1415-4757     ISO Abbreviation:  Genet. Mol. Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-28     Completed Date:  2012-12-31     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  100883590     Medline TA:  Genet Mol Biol     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  741-2     Citation Subset:  -    
Affiliation:
Department of Genetics, Universidade Federal de Paraná, Curitiba, PR, Brazil.
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Journal Information
Journal ID (nlm-ta): Genet Mol Biol
Journal ID (iso-abbrev): Genet. Mol. Biol
Journal ID (publisher-id): GMB
ISSN: 1415-4757
ISSN: 1678-4685
Publisher: Sociedade Brasileira de Genética, Ribeirão Preto, SP, Brazil
Article Information
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Copyright © 2012, Sociedade Brasileira de Genética.
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Received Day: 08 Month: 5 Year: 2012
Accepted Day: 20 Month: 7 Year: 2012
Print publication date: Month: 12 Year: 2012
Electronic publication date: Day: 09 Month: 10 Year: 2012
Volume: 35 Issue: 4
First Page: 741 Last Page: 742
PubMed Id: 23271933
ID: 3526080
DOI: 10.1590/S1415-47572012005000063
Publisher Id: gmb-35-741

Effects of physical exercise on butyrylcholinesterase in obese adolescents
Isabela M.W. Silva1
Neiva Leite2
Dellyana Boberg1
Thais J. Chaves1
Gerusa M. Eisfeld2
Gisele M. Eisfeld2
Gleyse F. Bono1
Ricardo L.R. Souza1
Lupe Furtado-Alle1
1Department of Genetics, Universidade Federal de Paraná, Curitiba, PR, Brazil.
2Department of Physical Education, Universidade Federal de Paraná, Curitiba, PR, Brazil.
Correspondence: Send correspondence to Lupe Furtado Alle. Departamento de Genética, Universidade Federal do Paraná, Caixa Postal 81531-980, 19071 Curitiba, PR, Brazil. E-mail: lupealle@gmail.com.

Butyrylcholinesterase (BChE; EC 3.1.1.8) is coded by BCHE gene (3q26.1–q26.2), synthesized in the liver and distributed to several parts of the organism. Plasmatic BChE is found in four possible homomeric forms (G1 monomers, G2 dimmers, G3 trimers and G4 tetramers) or heteromeric forms formed in association with other proteins, such as albumin, G1-Alb (Masson, 1989).

Several studies verified that BChE has a role in lipid metabolism (Kutty et al., 1977), and is correlated with weight (Chautard-Freire-Maia et al., 1991; Li et al., 2008) and body mass index (Alcântara et al., 2001, 2003; Souza et al., 2005; Furtado-Alle et al., 2008). It is also known that plasma BChE activity is positively correlated with obesity (Kutty, 1980; Chautard-Freire-Maia et al., 1991; Furtado-Alle et al., 2008).

The aim of this study was to compare the relative intensity (RI) of BChE isoforms revealed as bands (G1, G1-Alb, G2 and G4) in obese adolescents before and after 12 weeks of physical exercise (PE), and to search for a correlation between RI of BChE isoform bands, plasma BChE activity and PE.

The sample comprised 54 obese adolescents (BMI above percentile 95 and mean age 12.6 ± 2.01), these being participants of a 12 week program of physical exercise. Aerobic exercise consisted of 50 to 100 min activity during the first four weeks. Intensity was set at 35%-55% of VO2 peak, and was increased to 55%–75% during the next eight weeks. Plasma was sampled at baseline and after terminating the program. A sample of non-obese adolescents (N = 45; mean age 13.3 ± 2.15) was used to measure plasma BChE activity.

The detection and analysis of BChE bands in plasma was made according to Boberg et al. (2010). Samples without any detectable BChE band were excluded from the analysis. Plasma BChE activity was measured according to Dietz et al. (1972), as modified by Evans and Wroe (1978). This study was approved by the Ethics Committee of Biological Sciences Sector from Federal University of Parana (05/2009).

Mean plasma BChE activity was significantly reduced after the 12 weeks program (before: 7.66 ± 2.64 KU/L, after: 5.89 ± 2.34 KU/L; t = 2.96, p = 0.008). Accompanying BChE activity, waist circumference (WC; before: 97.41 ± 11.20 cm, after: 94.62 ± 10.51 cm, t = 3.6 and p = 0.03), LDL-cholesterol (LDL-C; before: 94.45 ± 20.83 mg/dL, after: 86.00 ± 16.37 mg/dL, t = 2.77 and p = 0.012) and triglycerides (TG; before: 114.30 ± 57.14 mg/dL, after: 82.75 ± 42.66 mg/dL, t = 3.1 and p = 0.006) also showed significant reduction with PE, while HDL cholesterol (HDL-C) showed a significant increase (before: 42.54 ± 8.33 mg/dL, after: 49.05 ± 8.61 mg/dL; t = −4.53 and p = 0.0002). These changes in BChE activity, WC and lipid profile (LDL-C, TG and HDL-C) are consistent with an already described association between BChE and lipid metabolism. Considering that mean BChE activity in obese adolescents after the program was similar to that seen in non-obese adolescents (5.0 ± 0.11 KU/L, t = 1.5 and p = 0.13), and that mean BChE activity of each band was significantly reduced by PE (Table 1), the 12 weeks of PE may have led to a physiological regularization of plasmatic BChE activity. This contrasts with the lack of significance between means of RI of BChE isoform bands before and after the program (Table 1).

Boberg et al. (2010) had reported that higher plasma BChE activity is found in obese persons, the RI of each isoform is nonetheless maintained, independent of obesity. Our present data are in accordance with the observation made by Boberg et al. (2010), showing that, although mean BChE activity (total and related to each band, Table 1) decreased in response to PE, the RI of each band was maintained. This indicates that this proportionality is regulated and may therefore be important for BChE functions.


Notes

Associate Editor: Mara Hutz

Grants and scholarships were received from Coordenação de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) and Fundação Araucaria.


References
Alcântara VM,Rodrigues LC,Oliveira LC,Chautard-Freire-Maia EA. Year: 2001Association of the CHE2 locus with body mass index and butyrylcholinesterase activityHum Biol7358759511512685
Alcântara VM,Oliveira LC,Réa RR,Suplicy HL,Chautard-Freire-Maia EA. Year: 2003Butyrylcholinesterase and obesity in individuals with the CHE2 C5+ and CHE2 C5− phenotypesInt J Obes2715571564
Boberg D,Furtado-Alle L,Souza RLR,Chautard-Freire-Maia EA. Year: 2010Molecular forms of butyrylcholinesterase and obesityGenet Mol Biol3345245421637414
Chautard-Freire-Maia EA,Primo-Parmo SL,Picheth G,Lourenço MAC,Vieira MM. Year: 1991The C5 isozyme of serum cholinesterase and adult weightHum Hered413303391778609
Dietz AA,Rubinstein HM,Lubrano TE,Hodges LK. Year: 1972Improved method for the differentiation of cholinesterase variantsAm J Hum Genet2458645012693
Evans RT,Wroe J. Year: 1978Is serum cholinesterase activity a predictor of succinyl choline sensitivity? An assessment of four methodsClin Chem2417621766699286
Furtado-Alle L,Andrade FA,Nunes K,Mikami LR,Souza RLR,Chautard-Freire-Maia EA. Year: 2008Association of variants of the −116 site of the butyrylcholinesterase BCHE gene to enzyme activity and body mass indexChem Biol Interact17511511818550040
Kutty KM. Year: 1980Biological function of cholinesteraseClin Biochem13239437011592
Kutty KM,Redheendran R,Murphy D. Year: 1977Serum cholinesterase: Function in lipoprotein metabolismExperientia33420421193712
Li B,Duysen EG,Lockridge O. Year: 2008The butyrylcholinesterase knockout mouse is obese on a high-fat dietChem Biol Interact175889118452903
Masson P. Year: 1989A naturally occurring molecular form of human plasma cholinesterase is an albumin conjugateBiochim Biophys Acta9882582662553123
Souza RLR,Fadel-Picheth C,Allebrandt KV,Furtado L,Chautard-Freire-Maia EA. Year: 2005Possible influence of BCHE locus of butyrylcholinesterase on stature and body mass indexAm J Phys Anthropol12632933415386241

Article Categories:
  • Human and Medical Genetics
    • Short Communication

Keywords: BChE activity, physical exercise, obesity.

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