| Effects of phosphorylation-related drugs on slow Ca2+ tail current in guinea-pig detrusor cells. | |
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MedLine Citation:
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PMID: 10323268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In isolated guinea-pig detrusor cells, large conditioning depolarizations evoke slowly deactivating Ca2+ tail currents, considered to represent the second open state. The possible involvement of channel phosphorylation in this open state was examined. Application of isoprenaline caused a marginal increase in Ca2+ channel current evoked by simple depolarization, while forskolin did not. During application of either drug, slow-tail currents were never observed after simple depolarizations. The conditions necessary to induce slow-tail currents were not changed, even when cyclic AMP, ATP-gamma-S (adenosine 5'-O-(3-thiotriphosphate)), GDP-beta-S (guanosine 5'-O-(2-thiodiphosphate)) (in the pipette) or H-7 (1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride) (to the bathing solution) was applied. The frequent depolarization protocol, known to facilitate Ca2+ current via Ca2+ and cyclic AMP-dependent phosphorylation mechanism(s) in cardiac myocytes, did not induce slow-tail currents. These results suggest that the transition of Ca2+ channels to the second open state during large depolarization is not a result of (voltage-operated) channel phosphorylation itself. Possible underlying mechanisms are discussed. |
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Authors:
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L M Smith; S Kajioka; A F Brading; S Nakayama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of pharmacology Volume: 370 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-07-16 Completed Date: 1999-07-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 187-93 Citation Subset: IM |
Affiliation:
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Department of Physiology, School of Medicine, Nagoya University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenergic beta-Agonists
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pharmacology Animals Calcium Channels / drug effects*, physiology Cyclic AMP / pharmacology Electric Stimulation Female Guinea Pigs Isoproterenol / pharmacology Male Membrane Potentials / drug effects Muscle, Smooth / drug effects*, physiology Patch-Clamp Techniques Phosphorylation Urinary Bladder / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic beta-Agonists; 0/Calcium Channels; 60-92-4/Cyclic AMP; 7683-59-2/Isoproterenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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