| Effects of phosphorothioate anti-sense oligodeoxynucleotides on colorectal cancer cell growth and telomerase activity. | |
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MedLine Citation:
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PMID: 15526364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the inhibitory effect of phosphorothioate anti-sense oligodeoxynucleotides (PASODN) on colorectal cancer LS-174T cells in vitro and the mechanism of inhibition of telomerase activity in these cells. METHODS: PASODN were used to infect LS-174T cells and block human telomerase RNA (hTR) through anti-sense technology. The inhibitory effect of PASODN was evaluated by colony-forming inhibition assay and growth curve. Changes of telomerase activity in LS-174T cells were detected by polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA), and the level of apoptosis was analyzed by flow cytometry (FCM) assay. RESULTS: PASODN showed a dose and time-dependent inhibition of cell proliferation. The optimal dosage of PASODN was 10 mumol/L. The colony-forming efficiency was 10.3% in PASODN group after 10 d, whereas that in phosphorothioate mis-sense oligodeoxynucleotides (PMSODN) group with the same concentration and in PBS group (blank control) was 49.1% and 50.7%, respectively. PCR-ELISA results indicated that telomerase activity in the PASODN group was obviously inhibited in comparison with in the control groups (P<0.01, t = 3.317 and 3.241, t0.01(20) = 2.845). Meanwhile, before the number of cells was decreased, the morphological changes were observed in the cells of PASODN group. The cells in PASODN group showed the apoptotic peak at 72 h after infection, whereas the control group did not show. CONCLUSION: Specific sequence oligonucleotides can inhibit telomerase activity and lead to cell apoptosis, suggesting a novel treatment strategy for malignant tumors induced by telomerase. |
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Authors:
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Xi-Shan Wang; Kuan Wang; Xue Li; Song-Bin Fu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 10 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-04 Completed Date: 2005-01-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 3455-8 Citation Subset: IM |
Affiliation:
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Department of Abdominal Surgery, Tumour Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China. 1966120808@sina.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis Cell Division Cell Line, Tumor / cytology Colorectal Neoplasms* Enzyme Activation / genetics Flow Cytometry Humans Neoplastic Stem Cells Oligodeoxyribonucleotides, Antisense / pharmacology* Telomerase / metabolism* Thionucleotides / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Oligodeoxyribonucleotides, Antisense; 0/Thionucleotides; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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