Document Detail


Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen.
MedLine Citation:
PMID:  11695757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to evaluate the effects of phosphoric acid (PA) and a proprietary glutaraldehyde-HEMA aqueous solution (Gluma Desensitizer; GD) on dentin collagen. Specimens of demineralized bovine dentin collagen were treated with either 37% or 50% PA for 1 or 5 min. An additional set of specimens was treated with 37% PA for 1 min followed by GD for 1 min. All specimens were washed with distilled water, lyophilized. reduced with standardized NaB3H4, hydrolyzed with 6 M HCl and subjected to amino acid and cross-link analyses. The results demonstrated that the treatment of demineralized dentin with PA under the conditions tested did not significantly alter the collagen cross-links. The GD-treated samples showed reduction of free lysine (Lys) and hydroxylysine (Hyl) residues, as well as a decrease in the levels of collagen reducible cross-links. In addition, unidentified reducible compounds were detected by high-performance liquid chromatography (HPLC) analysis. These compounds may be derived from cross-links formed between GD-derived aldehyde and Lys/Hyl of collagen. The findings indicate that PA treatment does not significantly affect dentin collagen amino acid and cross-link composition, and that GD treatment affects dentin collagen amino acid and cross-link composition.
Authors:
A V Ritter; E J Swift; M Yamauchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  European journal of oral sciences     Volume:  109     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-11-06     Completed Date:  2002-02-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  348-53     Citation Subset:  D; IM    
Affiliation:
Department of Operative Dentistry, University of North Carolina at Chapel Hill, 27599-7455, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Analysis of Variance
Animals
Cattle
Chromatography, High Pressure Liquid
Collagen / chemistry,  drug effects*
Dentin / drug effects*
Freeze Drying
Glutaral / pharmacology*
Hydrolysis
Hydroxylysine / analysis,  chemistry
Hydroxyproline / analysis,  chemistry
Lysine / analysis,  chemistry
Methacrylates / pharmacology*
Oxidation-Reduction
Phosphoric Acids / pharmacology*
Statistics as Topic
Grant Support
ID/Acronym/Agency:
DE10489/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Methacrylates; 0/Phosphoric Acids; 0/gluma desensitizer; 111-30-8/Glutaral; 28902-93-4/Hydroxylysine; 51-35-4/Hydroxyproline; 56-87-1/Lysine; 868-77-9/hydroxyethyl methacrylate; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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