| Effects of phalloidin microinjection and localization of fluorescein-labeled phalloidin in living sand dollar eggs. | |
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MedLine Citation:
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PMID: 7172218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Effects of microinjection of phalloidin on fertilization and cleavage of sand dollar (Clypeaster japonicus and Scaphechinus mirabilis) eggs were studied. The drug, previously injected into unfertilized eggs, showed no effect on the elevation of the fertilization membrane upon insemination up to an intracellular concentration of 50 microM. However, the movement of the egg pronucleus to the sperm pronucleus was inhibited and the fusion of pronuclei did not occur. The subsequent development no longer took place. When phalloidin was injected into fertilized eggs, the thickness of the cortical layer increased and the microvilli became conspicuous. Both nuclear division and cleavage were inhibited at the intracellular concentration of more than 20 microM, though the latter seemed to be more sensitive to phalloidin than the former. Fluorescein-labeled phalloidin (FL-phalloidin) was injected into eggs in order to investigate F-actin localization by fluorescence microscopy. In both unfertilized and fertilized eggs, FL-phalloidin was localized in the cortical layer within 1 min after injection. It was also localized in the cortical layer as radially oriented rod-like structures when injected into fertilized eggs before the disappearance of the nuclear membrane. No distinct fluorescence was detected in the mitotic apparatus or in the cleavage furrow. FL-phalloidin redistributed gradually into egg cytoplasm. In unfertilized eggs, fluorescent rods were found especially in the egg pronucleus 30 min after injection. |
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Authors:
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Y Hamaguchi; I Mabuchi |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell motility Volume: 2 ISSN: 0271-6585 ISO Abbreviation: Cell Motil. Publication Date: 1982 |
Date Detail:
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Created Date: 1983-02-25 Completed Date: 1983-02-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8207421 Medline TA: Cell Motil Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 103-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Cell Nucleus / metabolism Cleavage Stage, Ovum / drug effects Female Fertilization / drug effects Fluoresceins Microinjections Oligopeptides / pharmacology* Ovum / drug effects*, metabolism Phalloidine / metabolism, pharmacology* Sea Urchins |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Fluoresceins; 0/Oligopeptides; 17466-45-4/Phalloidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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