| Effects of perfluorocarbon liquids and silicone oil on human retinal pigment epithelial cells and retinal ganglion cells. | |
| | |
MedLine Citation:
|
PMID: 19174720 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To examine the effects of perfluorocarbon liquid (PFCL) and silicone oil (SO) on human retinal pigment epithelium (RPE) cells and retinal ganglion cells (RGCs) in vitro. METHODS: Human RPE cells (ARPE-19 cells), seeded on microporous inserts, were exposed to PFCL or SO and incubated for 3 or 7 days. Perfluorocarbon liquid was in contact with cells at the apical or baso-lateral side, not inhibiting cell feeding. Then, the quantification of cell proliferation and cell viability were evaluated by WST-1 assay. In the same way, RGCs were exposed for 1 hour or 3 days, and the number of viable RGCs was counted by using a fluorescence viability agent. RESULTS: Perfluorocarbon liquid affected the survival of ARPE-19 cells and RGCs when compared with the nontreated control group. ARPE-19 cells decreased significantly after being in contact with PFCL at the baso-lateral side for 7 days. However, PFCL contact at the apical side reduced the number of RGCs in a time-dependent manner. In case of SO, the viability of the ARPE-19 cells decreased significantly after being in contact with SO at the baso-lateral side for 7 days. However, SO did not reduce the number of RGCs after a 3-day exposure. CONCLUSION: Perfluorocarbon liquid is directly toxic to ARPE-19 cells when exposed to the cells for 7 days. On the contrary, it seems that RGCs are damaged in a time-dependent manner by the more mechanical rather than toxic effects of PFCL. Silicone oil seems to exert mechanical rather than toxic effects on ARPE-19 cells. When PFCL is used as a postoperative tamponade clinically, understanding the difference in the effects will lead to more effective and safer results. |
| | |
Authors:
|
Maiko Inoue; Aya Iriyama; Kazuaki Kadonosono; Yasuhiro Tamaki; Yasuo Yanagi |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Retina (Philadelphia, Pa.) Volume: 29 ISSN: 1539-2864 ISO Abbreviation: Retina (Philadelphia, Pa.) Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-05-11 Completed Date: 2009-06-04 Revised Date: 2010-03-17 |
Medline Journal Info:
|
Nlm Unique ID: 8309919 Medline TA: Retina Country: United States |
Other Details:
|
Languages: eng Pagination: 677-81 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, Yokohama City University Medical Center, Yokohama, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Fluorocarbons / toxicity* Humans Rats Rats, Wistar Retinal Ganglion Cells / drug effects* Retinal Pigment Epithelium / drug effects* Silicone Oils / toxicity* Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Fluorocarbons; 0/Silicone Oils; 307-34-6/perfluorooctane |
| Comments/Corrections | |
Comment In:
|
Retina. 2010 Mar;30(3):534-5; author reply 535-6
[PMID:
20216296
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Association of the extent of diabetic macular edema as assessed by optical coherence tomography with...
Next Document: Vital dyes for macular surgery: a comparative electron microscopy study of the internal limiting mem...