Document Detail

Effects of perfluorocarbon liquids and silicone oil on human retinal pigment epithelial cells and retinal ganglion cells.
MedLine Citation:
PMID:  19174720     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To examine the effects of perfluorocarbon liquid (PFCL) and silicone oil (SO) on human retinal pigment epithelium (RPE) cells and retinal ganglion cells (RGCs) in vitro. METHODS: Human RPE cells (ARPE-19 cells), seeded on microporous inserts, were exposed to PFCL or SO and incubated for 3 or 7 days. Perfluorocarbon liquid was in contact with cells at the apical or baso-lateral side, not inhibiting cell feeding. Then, the quantification of cell proliferation and cell viability were evaluated by WST-1 assay. In the same way, RGCs were exposed for 1 hour or 3 days, and the number of viable RGCs was counted by using a fluorescence viability agent. RESULTS: Perfluorocarbon liquid affected the survival of ARPE-19 cells and RGCs when compared with the nontreated control group. ARPE-19 cells decreased significantly after being in contact with PFCL at the baso-lateral side for 7 days. However, PFCL contact at the apical side reduced the number of RGCs in a time-dependent manner. In case of SO, the viability of the ARPE-19 cells decreased significantly after being in contact with SO at the baso-lateral side for 7 days. However, SO did not reduce the number of RGCs after a 3-day exposure. CONCLUSION: Perfluorocarbon liquid is directly toxic to ARPE-19 cells when exposed to the cells for 7 days. On the contrary, it seems that RGCs are damaged in a time-dependent manner by the more mechanical rather than toxic effects of PFCL. Silicone oil seems to exert mechanical rather than toxic effects on ARPE-19 cells. When PFCL is used as a postoperative tamponade clinically, understanding the difference in the effects will lead to more effective and safer results.
Maiko Inoue; Aya Iriyama; Kazuaki Kadonosono; Yasuhiro Tamaki; Yasuo Yanagi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Retina (Philadelphia, Pa.)     Volume:  29     ISSN:  1539-2864     ISO Abbreviation:  Retina (Philadelphia, Pa.)     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-06-04     Revised Date:  2010-03-17    
Medline Journal Info:
Nlm Unique ID:  8309919     Medline TA:  Retina     Country:  United States    
Other Details:
Languages:  eng     Pagination:  677-81     Citation Subset:  IM    
Department of Ophthalmology, Yokohama City University Medical Center, Yokohama, Japan.
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MeSH Terms
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Fluorocarbons / toxicity*
Rats, Wistar
Retinal Ganglion Cells / drug effects*
Retinal Pigment Epithelium / drug effects*
Silicone Oils / toxicity*
Time Factors
Reg. No./Substance:
0/Fluorocarbons; 0/Silicone Oils; 307-34-6/perfluorooctane
Comment In:
Retina. 2010 Mar;30(3):534-5; author reply 535-6   [PMID:  20216296 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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