Document Detail


Effects of perfluorinated amphiphiles on backward swimming in Paramecium caudatum.
MedLine Citation:
PMID:  16300727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PFOS and PFOA are ubiquitous contaminants in the environment. We investigated the effects of fluorochemicals on calcium currents in Paramecium caudatum using its behavioral changes. Negatively charged amphiphiles prolonged backward swimming (BWS) of Paramecium. PFOS significantly prolonged BWS, while PFOA was less potent (EC(50): 29.8+/-4.1 and 424.1+/-124.0microM, respectively). The BWS prolongation was blocked by cadmium, indicating that the cellular calcium conductance had been modified. The positively charged amphiphile FOSAPrTMA shortened BWS (EC(50): 19.1+/-17.3). Nonionic amphiphiles did not affect BWS. The longer-chain perfluorinated carboxylates PFNA and PFDA were more potent than PFOA (EC(50): 98.7+/-20.1 and 60.4+/-10.1microM, respectively). However, 1,8-perfluorooctanedioic acid and 1,10-perfluorodecanedioic acid did not prolong BWS. The critical micelle concentration (CMC) and BWS prolongation for negatively charged amphiphiles showed a clear correlation (r(2)=0.8008, p<0.001). In summary, several perfluorochemicals and PFOS and PFOA had similar effects in Paramecium, while chain length, CMC, and electric charge were major determinants of BWS duration.
Authors:
Eriko Matsubara; Kouji Harada; Kayoko Inoue; Akio Koizumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-16
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  339     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-07     Completed Date:  2006-02-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  554-61     Citation Subset:  IM    
Affiliation:
Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 6068501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkanesulfonic Acids / chemistry,  pharmacology*
Animals
Cadmium / pharmacology
Carbon / chemistry
Fluorine / chemistry*
Fluorocarbons / chemistry,  pharmacology*
Molecular Structure
Nifedipine / pharmacology
Octanoic Acids / chemistry,  pharmacology*
Paramecium caudatum / drug effects*,  physiology*
Structure-Activity Relationship
Swimming / physiology*
Chemical
Reg. No./Substance:
0/Alkanesulfonic Acids; 0/Fluorocarbons; 0/Octanoic Acids; 1763-23-1/perfluorooctane sulfonic acid; 21829-25-4/Nifedipine; 335-67-1/perfluorooctanoic acid; 7440-43-9/Cadmium; 7440-44-0/Carbon; 7782-41-4/Fluorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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