| Effects of pepsin and trypsin on the anti-adipogenic action of lactoferrin against pre-adipocytes derived from rat mesenteric fat. | |
| | |
MedLine Citation:
|
PMID: 20854698 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Lactoferrin (LF) is a multifunctional glycoprotein in mammalian milk. In a previous report, we showed that enteric-coated bovine LF tablets can decrease visceral fat accumulation, hypothesising that the enteric coating is critical to the functional peptides reaching the visceral fat tissue and exerting their anti-adipogenic activity. The aim of the present study was to assess whether ingested LF can retain its anti-adipogenic activity. We therefore investigated the effects of LF and LF treated with digestive enzymes (the stomach enzyme pepsin and the small intestine enzyme trypsin) on lipid accumulation in pre-adipocytes derived from the mesenteric fat tissue of male Sprague-Dawley rats. Lipid accumulation in pre-adipocytes was significantly reduced by LF in a dose-dependent manner and was associated with reduction in gene expression of CCAAT/enhancer binding protein delta, CCAAT/enhancer binding protein alpha and PPARγ as revealed by DNA microarray analysis. Trypsin-treated LF continued to show anti-adipogenic action, whereas pepsin-treated LF abrogated the activity. When an LF solution (1000 mg bovine LF) was administered by gastric intubation to Sprague-Dawley rats, immunoreactive LF determined by ELISA could be detected in mesenteric fat tissue at a concentration of 14·4 μg/g fat after 15 min. The overall results point to the importance of enteric coating for action of LF as a visceral fat-reducing agent when administered in oral form. |
| | |
Authors:
|
Tomoji Ono; Satoru Morishita; Chikako Fujisaki; Motoyasu Ohdera; Michiaki Murakoshi; Norio Iida; Hisanori Kato; Kazuo Miyashita; Masaaki Iigo; Toshihide Yoshida; Keikichi Sugiyama; Hoyoku Nishino |
Related Documents
:
|
8653528 - Long-term weight cycling in female wistar rats: effects on metabolism. 10877198 - Effects of a high-fat-sucrose diet on enzymes in homocysteine metabolism in the rat. 20978528 - Gastrointestinal transit, post-prandial lipaemia and satiety following 3 days high-fat ... 22117618 - Effects of lifestyle intervention in persons at risk for type 2 diabetes mellitus--resu... 7254458 - Tetratological evaluation of mouse fetuses after paternal alcohol ingestion. 6453958 - Effect of diet on plasma taurine in the cat. |
Publication Detail:
|
Type: Journal Article Date: 2010-09-21 |
Journal Detail:
|
Title: The British journal of nutrition Volume: 105 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-01-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
|
Languages: eng Pagination: 200-11 Citation Subset: IM |
Affiliation:
|
Research and Development Headquarters, Lion Corporation, 100 Tajima, Odawara, Kanagawa 256-0811, Japan. tomoono@lion.co.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome.
Next Document: Fermentation of calcium-fortified soya milk does not appear to enhance acute calcium absorption in o...