Document Detail

Effects of pentachlorophenol and 2,4,6-trichlorophenol on the disposition of sulfobromophthalein and respiration of isolated liver cells.
MedLine Citation:
PMID:  7387397     Owner:  NLM     Status:  MEDLINE    
The effect of pentachlorophenol (PCP) and 2,4,6-tricholorphenol (2,4,6-T) on the disposition of the hepatodiagnostic dye, sulfobromophthalein (BSP) has been studied in isolated liver cells. PCP (4-6 microM) as well as 2,4,6-T (50-100 microM) interferes with the disposition of BSP. The main effect apparently occurs at the secretion step as both drugs severely impair the release of the glutathione conjugate of BSP into the medium. As a consequence, BSP and its conjugate accumulate in the cell. High doses of PCP did not increase the release of lactate dehydrogenase from the hepatocytes. Concentrations of the two phenols which interfere with the secretion of BSP also completely uncouple the oxidative phosphorylation of hepatocellular mitochondria. The dysfunction of liver cells described here may therefore be explained by the effect of PCP and 2,4,6-T on the energy production of the cells. The higher toxicity of PCP as compared to 2,4,6-T observed in our system corresponds well with the higher LD50 of the latter compound.
R Götz; L R Schwarz; H Greim
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Archives of toxicology     Volume:  44     ISSN:  0340-5761     ISO Abbreviation:  Arch. Toxicol.     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-08-25     Completed Date:  1980-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  147-55     Citation Subset:  IM    
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MeSH Terms
Chlorophenols / pharmacology*
L-Lactate Dehydrogenase / metabolism
Liver / cytology,  enzymology,  metabolism*
Mitochondria, Liver / metabolism
Oxygen Consumption / drug effects*
Pentachlorophenol / pharmacology*
Pesticides / pharmacology*
Sulfobromophthalein / metabolism*
Time Factors
Reg. No./Substance:
0/Chlorophenols; 0/Pesticides; 297-83-6/Sulfobromophthalein; 87-86-5/Pentachlorophenol; 88-06-2/2,4,6-trichlorophenol; EC Dehydrogenase

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