Document Detail


Effects of pentachlorophenol and 2,4,6-trichlorophenol on the disposition of sulfobromophthalein and respiration of isolated liver cells.
MedLine Citation:
PMID:  7387397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of pentachlorophenol (PCP) and 2,4,6-tricholorphenol (2,4,6-T) on the disposition of the hepatodiagnostic dye, sulfobromophthalein (BSP) has been studied in isolated liver cells. PCP (4-6 microM) as well as 2,4,6-T (50-100 microM) interferes with the disposition of BSP. The main effect apparently occurs at the secretion step as both drugs severely impair the release of the glutathione conjugate of BSP into the medium. As a consequence, BSP and its conjugate accumulate in the cell. High doses of PCP did not increase the release of lactate dehydrogenase from the hepatocytes. Concentrations of the two phenols which interfere with the secretion of BSP also completely uncouple the oxidative phosphorylation of hepatocellular mitochondria. The dysfunction of liver cells described here may therefore be explained by the effect of PCP and 2,4,6-T on the energy production of the cells. The higher toxicity of PCP as compared to 2,4,6-T observed in our system corresponds well with the higher LD50 of the latter compound.
Authors:
R Götz; L R Schwarz; H Greim
Related Documents :
3349467 - In vivo selection of highly metastatic cells from surgical specimens of different prima...
10934157 - Liver damage using suicide genes. a model for oval cell activation.
19725107 - The niche of stellate cells within rat liver.
7532877 - Growth kinetics of enzyme-altered liver foci in rats treated with phenobarbital or alph...
910577 - Stimulation of reticuloendothelial system and toxicity to macrophages of staphylococcus...
18434687 - Association of high proliferation in adult t-cell leukemia cells with apoptosis, and ex...
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Archives of toxicology     Volume:  44     ISSN:  0340-5761     ISO Abbreviation:  Arch. Toxicol.     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-08-25     Completed Date:  1980-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  147-55     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Chlorophenols / pharmacology*
L-Lactate Dehydrogenase / metabolism
Liver / cytology,  enzymology,  metabolism*
Male
Mitochondria, Liver / metabolism
Oxygen Consumption / drug effects*
Pentachlorophenol / pharmacology*
Pesticides / pharmacology*
Rats
Sulfobromophthalein / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Chlorophenols; 0/Pesticides; 297-83-6/Sulfobromophthalein; 87-86-5/Pentachlorophenol; 88-06-2/2,4,6-trichlorophenol; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparative studies on the uptake of 14C-bile acids and 3H-demethylphalloin in isolated rat liver ce...
Next Document:  Thymidine transport in hepatocytes. An assay for hepatotoxicity.