Document Detail


Effects of a pectic polysaccharide from a medicinal herb, the roots of Bupleurum falcatum L. on interleukin 6 production of murine B cells and B cell lines.
MedLine Citation:
PMID:  10996028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of T-cell-independent B cell mitogen, a pectic polysaccharide, bupleuran 2IIc, from a medicinal herb, the roots of Bupleurum falcatum L. on interleukin 6 (IL-6) production of murine B cells and B cell lines was investigated in order to clarify the mechanism of enhanced immunoglobulin (Ig) secretion from B cells. Bupleuran 2IIc enhanced IgM secretion from highly purified murine normal B cells. When normal B cells from murine spleen were cultured with bupleuran 2IIc in the presence of anti-IL-6 neutralizing antibody, the enhanced IgM secretion by bupleuran 2IIc was reduced. When B cells were stimulated with bupleuran 2IIc, their IL-6 secretion and the transcription of IL-6 mRNA were enhanced. The enhanced IL-6 secretion by bupleuran 2IIc was also observed in B cell line, Y16 cell. When bupleuran 2IIc was digested with endo-alpha-(1-->4)-D-polygalacturonase, the resulting enzyme resistant carbohydrate portion, "ramified" region (PG-1) composed of rhamnogalacturonan core containing neutral sugar side chains showed potent IL-6 secretion-enhancing activity. These results suggest that the "ramified" region of bupleuran 2IIc stimulates the secretion of IL-6 as the active site, and the resulting IL-6 may partially contribute the enhancement of IgM secretion as an autocrine and/or paracrine mechanism.
Authors:
Y Guo; T Matsumoto; Y Kikuchi; T Ikejima; B Wang; H Yamada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunopharmacology     Volume:  49     ISSN:  0162-3109     ISO Abbreviation:  Immunopharmacology     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-15     Completed Date:  2001-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902474     Medline TA:  Immunopharmacology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  307-16     Citation Subset:  IM    
Affiliation:
Oriental Medicine Research Center, The Kitasato Institute, 5-9-1, Shirokane, Minato-ku, 108-8642, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / chemistry,  pharmacology*
Animals
B-Lymphocytes / drug effects*,  secretion
Bupleurum
Cell Line
Drugs, Chinese Herbal / chemistry,  pharmacology*
Female
Immune Sera / pharmacology
Immunoglobulin M / biosynthesis
Interleukin-6 / biosynthesis*,  immunology,  secretion
Mice
Mice, Inbred C3H
Pectins / chemistry,  pharmacology*
Plant Extracts / chemistry,  pharmacology*
Plant Roots / chemistry
Plants, Medicinal / chemistry*
Structure-Activity Relationship
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Bupleurum root extract; 0/Drugs, Chinese Herbal; 0/Immune Sera; 0/Immunoglobulin M; 0/Interleukin-6; 0/Pectins; 0/Plant Extracts; 138726-08-6/Bupleuran 2IIc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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