Document Detail


Effects of panduratin A isolated from Kaempferia pandurata ROXB. on the expression of matrix metalloproteinase-9 by porphyromonas gingivalis supernatant-induced KB cells.
MedLine Citation:
PMID:  19122290     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Periodontitis is initiated by accumulation of microbial plaque and activation of gingival inflammation through overexpression of matrix metalloproteinases (MMPs), leading to tissue destruction. Natural MMP inhibitors may be developed as therapeutic agents against periodontitis. In this study, panduratin A, a natural bioactive compound isolated from Kaempferia pandurata ROXB., was used to test its in vitro inhibitory activity against MMP-9 secretion from Porphyromonas gingivalis supernatant-induced human oral epidermoid carcinoma KB cells. Gelatin zymography, Western blot and RT-PCR analyses were performed to evaluate MMP-9 expression. The gelatin zymograms revealed that the main gelatinase secreted by P. gingivalis supernatant-induced KB cells migrated at 92 kDa, representing MMP-9. MMP-9 protein and mRNA levels were significantly decreased after panduratin A treatment (p<0.05). In contrast, panduratin A had no effect on tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2 mRNA. Panduratin A also suppressed urokinase type plasminogen activator (uPA) mRNA expression. These results suggest that panduratin A could potentially prevent periodontal inflammation by decreasing the levels of MMP-9 protein and mRNA.
Authors:
Yanti; Hyun-In Oh; Anggakusuma; Jae-Kwan Hwang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  32     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-05     Completed Date:  2009-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  110-5     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Yonsei University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma / metabolism
Cell Line, Tumor
Cell Survival / drug effects
Chalcones / pharmacology
Culture Media / pharmacology
Dose-Response Relationship, Drug
Drug Interactions
Gene Expression Regulation, Neoplastic / drug effects*
Humans
Matrix Metalloproteinase 9 / genetics,  metabolism*
Porphyromonas gingivalis / chemistry*
Zingiberaceae / chemistry*
Chemical
Reg. No./Substance:
0/Chalcones; 0/Culture Media; 0/panduratin A; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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