Document Detail

Effects of pH, nitrite, and ascorbic acid on nonenzymatic nitric oxide generation and bacterial growth in urine.
MedLine Citation:
PMID:  11730365     Owner:  NLM     Status:  MEDLINE    
Nitrite may be generated by bacteria in urine during urinary tract infections. Acidification of nitrite results in the formation of nitric oxide (NO) and other reactive nitrogen oxides, which are toxic to a variety of microorganisms. We have studied NO formation and bacterial growth in mildly acidified human urine containing nitrite and the reducing agent vitamin C. Urine collected from healthy subjects was incubated in closed syringes at different pH values with varying amounts of nitrite and/or ascorbic acid added. NO generation was measured in headspace gas using a chemiluminescence technique. A similar setup was also used to study the growth of three strains of bacteria in urine. Mildly acidified nitrite-containing urine generated large amounts of NO and this production was greatly potentiated by ascorbic acid. The growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus saprophyticus was markedly reduced by the addition of nitrite to acidified urine. This inhibition was enhanced by ascorbic acid. In conclusion, we show that the growth of three common urinary pathogens is markedly inhibited in mildly acidified urine when nitrite is present. The bacteriostatic effect of acidified nitrite is likely related to the release of NO and other toxic reactive nitrogen intermediates. These results may help to explain the well-known beneficial effects of urinary acidification with, e.g., vitamin C in treatment and prevention of urinary tract infection.
S Carlsson; N P Wiklund; L Engstrand; E Weitzberg; J O Lundberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society     Volume:  5     ISSN:  1089-8603     ISO Abbreviation:  Nitric Oxide     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-03     Completed Date:  2002-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709307     Medline TA:  Nitric Oxide     Country:  United States    
Other Details:
Languages:  eng     Pagination:  580-6     Citation Subset:  IM    
Department of Surgery, Section of Urology, Karolinska Hospital, Stockholm, Sweden.
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MeSH Terms
Ascorbic Acid / pharmacology*
Bacteriuria / microbiology*
Escherichia coli / growth & development
Hydrogen-Ion Concentration*
Middle Aged
Nitric Oxide / biosynthesis*
Nitrites / pharmacology*
Pseudomonas aeruginosa / growth & development
Staphylococcus / growth & development
Reg. No./Substance:
0/Nitrites; 10102-43-9/Nitric Oxide; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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