| Effects of pH on the conformation and activation of plasminogen. | |
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MedLine Citation:
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PMID: 4071469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glu-plasminogen (plg) or Lys-plg solution was kept at pH 2.0 for various time intervals, and then readjusted to pH 7.0. The activation rate and conformational changes of such acid-treated plg were measured using the hydrolysis of S-2251, spectrofluorometry and spectropolarimetry (circular dichroism). The activation rate of acid-treated Glu-plg increased after readjustment to pH 7.0. The increase was not reversible even after keeping acid-treated Glu-plg for 24 hrs at neutral pH. The activation rate of acid-treated Lys-plg rather decreased. The fluorescence intensity at 340 nm of acid-treated plg decreased, and the intensity of fluorescence induced by the interaction of plasminogen with ANS (1-anilino-8-naphthalene sulfonate) increased after keeping plasminogen for longer than 24 hrs at pH 2.0. Circular dichroism spectra indicated that the secondary and tertiary structure of Glu-plg changed after acidification, and that only the tertiary structure of Lys-plg changed. |
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Authors:
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A Takada; Y Takada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Thrombosis research Volume: 40 ISSN: 0049-3848 ISO Abbreviation: Thromb. Res. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1986-01-22 Completed Date: 1986-01-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0326377 Medline TA: Thromb Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 189-97 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anilino Naphthalenesulfonates Circular Dichroism Enzyme Activation Humans Hydrogen-Ion Concentration Plasminogen / metabolism* Protein Conformation Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Anilino Naphthalenesulfonates; 82-76-8/1-anilino-8-naphthalenesulfonate; 9001-91-6/Plasminogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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