Document Detail

Effects of pH on the conformation and activation of plasminogen.
MedLine Citation:
PMID:  4071469     Owner:  NLM     Status:  MEDLINE    
Glu-plasminogen (plg) or Lys-plg solution was kept at pH 2.0 for various time intervals, and then readjusted to pH 7.0. The activation rate and conformational changes of such acid-treated plg were measured using the hydrolysis of S-2251, spectrofluorometry and spectropolarimetry (circular dichroism). The activation rate of acid-treated Glu-plg increased after readjustment to pH 7.0. The increase was not reversible even after keeping acid-treated Glu-plg for 24 hrs at neutral pH. The activation rate of acid-treated Lys-plg rather decreased. The fluorescence intensity at 340 nm of acid-treated plg decreased, and the intensity of fluorescence induced by the interaction of plasminogen with ANS (1-anilino-8-naphthalene sulfonate) increased after keeping plasminogen for longer than 24 hrs at pH 2.0. Circular dichroism spectra indicated that the secondary and tertiary structure of Glu-plg changed after acidification, and that only the tertiary structure of Lys-plg changed.
A Takada; Y Takada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  40     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1986-01-22     Completed Date:  1986-01-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  189-97     Citation Subset:  IM    
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MeSH Terms
Anilino Naphthalenesulfonates
Circular Dichroism
Enzyme Activation
Hydrogen-Ion Concentration
Plasminogen / metabolism*
Protein Conformation
Spectrometry, Fluorescence
Reg. No./Substance:
0/Anilino Naphthalenesulfonates; 82-76-8/1-anilino-8-naphthalenesulfonate; 9001-91-6/Plasminogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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