Document Detail


Effects of oxymatrine on proliferation and apoptosis in human hepatoma cells.
MedLine Citation:
PMID:  16458489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxymatrine, a natural quinolizidine alkaloid, has been known having cytotoxic and chemopreventive effects on various cancer cells. To investigate the possible mechanism of oxymatrine's role on cancer cells, in the present study, we examined further the effects of oxymatrine on the growth, proliferation, apoptosis and expression of bcl-2 and p53 gene in human hepatoma SMMC-7721 cells in vitro. Our results show that oxymatrine notably inhibits the growth and proliferation of SMMC-7721 cells and it present a dose-dependence and time-dependence manner within definite reacting dose and time. Oxymatrine block SMMC-7721 cells in G2/M and S phase; prevent cells entering into G0/G1 phase. It results in an obvious accumulation of G2/M and S phase cells while decrease of G0/G1 phase cells. Oxymatrine induce apoptosis of SMMC-7721 cells and apoptotic rate amount to about 60% after treatment with 1.0 mg/ml oxymatrine for 48 h. We also find that oxymatrine down-regulate expression of bcl-2 gene while up-regulate expression of p53 gene. These results demonstrate that oxymatrine inhibit the proliferation and induce apoptosis of human hepatoma SMMC-7721 cells, and suggest that this effect was mediated probably by a significant cell cycle blockage in G2/M and S phase, down-regulation of bcl-2 and up-regulation of p53.
Authors:
Guanbin Song; Qing Luo; Jian Qin; Lu Wang; Yisong Shi; Caixin Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-03
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  48     ISSN:  0927-7765     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-06     Completed Date:  2006-04-20     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Affiliation:
College of Bioengineering, Chongqing University, Key Laboratory of Biomechanics and Tissue Engineering, State Ministry of Education, Chongqing 400044, PR China. song9973@tom.com
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / chemistry,  pharmacology*
Antineoplastic Agents / chemistry,  pharmacology*
Apoptosis / drug effects*
Carcinoma, Hepatocellular / drug therapy*,  metabolism
Cell Cycle / drug effects
Cell Division / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
Dose-Response Relationship, Drug
Down-Regulation / drug effects
G2 Phase
Gene Expression Regulation, Neoplastic / drug effects
Humans
Kinetics
Liver Neoplasms / drug therapy*,  metabolism
Molecular Conformation
Proto-Oncogene Proteins c-bcl-2 / metabolism
Quinolizines
S Phase
Time Factors
Tumor Suppressor Protein p53 / metabolism
Up-Regulation / drug effects
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Antineoplastic Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Quinolizines; 0/Tumor Suppressor Protein p53; 16837-52-8/oxymatrine

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