| Effects of oxymatrine on proliferation and apoptosis in human hepatoma cells. | |
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MedLine Citation:
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PMID: 16458489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxymatrine, a natural quinolizidine alkaloid, has been known having cytotoxic and chemopreventive effects on various cancer cells. To investigate the possible mechanism of oxymatrine's role on cancer cells, in the present study, we examined further the effects of oxymatrine on the growth, proliferation, apoptosis and expression of bcl-2 and p53 gene in human hepatoma SMMC-7721 cells in vitro. Our results show that oxymatrine notably inhibits the growth and proliferation of SMMC-7721 cells and it present a dose-dependence and time-dependence manner within definite reacting dose and time. Oxymatrine block SMMC-7721 cells in G2/M and S phase; prevent cells entering into G0/G1 phase. It results in an obvious accumulation of G2/M and S phase cells while decrease of G0/G1 phase cells. Oxymatrine induce apoptosis of SMMC-7721 cells and apoptotic rate amount to about 60% after treatment with 1.0 mg/ml oxymatrine for 48 h. We also find that oxymatrine down-regulate expression of bcl-2 gene while up-regulate expression of p53 gene. These results demonstrate that oxymatrine inhibit the proliferation and induce apoptosis of human hepatoma SMMC-7721 cells, and suggest that this effect was mediated probably by a significant cell cycle blockage in G2/M and S phase, down-regulation of bcl-2 and up-regulation of p53. |
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Authors:
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Guanbin Song; Qing Luo; Jian Qin; Lu Wang; Yisong Shi; Caixin Sun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-03 |
Journal Detail:
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Title: Colloids and surfaces. B, Biointerfaces Volume: 48 ISSN: 0927-7765 ISO Abbreviation: Colloids Surf B Biointerfaces Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-04-20 Revised Date: 2009-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-5 Citation Subset: IM |
Affiliation:
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College of Bioengineering, Chongqing University, Key Laboratory of Biomechanics and Tissue Engineering, State Ministry of Education, Chongqing 400044, PR China. song9973@tom.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaloids
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chemistry,
pharmacology* Antineoplastic Agents / chemistry, pharmacology* Apoptosis / drug effects* Carcinoma, Hepatocellular / drug therapy*, metabolism Cell Cycle / drug effects Cell Division / drug effects Cell Line, Tumor Cell Proliferation / drug effects* Dose-Response Relationship, Drug Down-Regulation / drug effects G2 Phase Gene Expression Regulation, Neoplastic / drug effects Humans Kinetics Liver Neoplasms / drug therapy*, metabolism Molecular Conformation Proto-Oncogene Proteins c-bcl-2 / metabolism Quinolizines S Phase Time Factors Tumor Suppressor Protein p53 / metabolism Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Alkaloids; 0/Antineoplastic Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Quinolizines; 0/Tumor Suppressor Protein p53; 16837-52-8/oxymatrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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