Document Detail

Effects of oxygen supply on electrical and secretory responses of humorally stimulated acinar cells in isolated rat pancreas.
MedLine Citation:
PMID:  966397     Owner:  NLM     Status:  MEDLINE    
A technique is described for perfusing isolated rat pancreas with a modified Krebs-Henseleit solution. This preparation made feasible the simultaneous measurement of transmembrane potential, effective membrane resistance, rate of flow of pancreatic juice and amylase output. The hyperpolarizing effect of cholecystokinin-pancreozymin (CCK-PZ) was confirmed by perfusion with a solution containing 5 mU CCK-PZ/ml. The hyperpolarizing efffect of CCK-PZ was enhanced when the pancreas was perfused with an oxygenated solution containing dog erythrocytes. This effect was inhibited by ouabain and anaerobic conditions. It is suggested that the oxidative metabolism supplies energy for a mechanism which is responsible for the hyperpolarizing effect of CCK-PZ. The amylase output and the rate of flow of pancreatic juice were enhanced concurrently under conditions that augumented the hyperpolarizing effect, and they were inhibited under conditions that suppressed the effect. Cellular events in stimulus-secretion coupling in the pancreatic acinar cell are discussed by correlating the electrophysiological responses and the secretory responses under various conditions.
T Kanno; T Suga; M Yamamoto
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Japanese journal of physiology     Volume:  26     ISSN:  0021-521X     ISO Abbreviation:  Jpn. J. Physiol.     Publication Date:  1976  
Date Detail:
Created Date:  1976-11-21     Completed Date:  1976-11-21     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  2985184R     Medline TA:  Jpn J Physiol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  101-15     Citation Subset:  IM    
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MeSH Terms
Amylases / metabolism*
Membrane Potentials*
Ouabain / pharmacology
Oxygen Consumption*
Pancreas / enzymology,  physiology*,  secretion
Pancreatic Juice / secretion*
Reg. No./Substance:
630-60-4/Ouabain; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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