| Effects of oxygen-free radicals on proliferation kinetics of cultured rabbit articular chondrocytes. | |
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MedLine Citation:
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PMID: 2808536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It can be postulated that among the factors implicated in cartilaginous lesions, oxygen-derived free radicals seem to have a prominent part. To investigate this hypothesis, rabbit articular chondrocyte cultures have been exposed to oxygen-derived reactive species generated by the hypoxanthine-xanthine oxydase system. We observed a dose-dependent decrease of cellular growth. In order to explain this result, cell cycle progression and binucleate cell fractions have been studied. A greater number of binucleate cells and an increase in cell volume were observed. Flow cytometry analysis revealed a perturbation in cell cycle progression leading to a significant increase in the proportion of cells in G2 phase and an important augmentation in cell protein content confirmed by biochemical assays. This model shows which type of alteration can be induced by oxygen-derived free radicals in vitro. In addition, we deem this model to be useful for studying degenerative processes and for screening drugs that can scavenge oxygen-free radicals. |
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Authors:
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F Vincent; H Brun; E Clain; X Ronot; M Adolphe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cellular physiology Volume: 141 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1989 Nov |
Date Detail:
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Created Date: 1989-12-01 Completed Date: 1989-12-01 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 262-6 Citation Subset: IM |
Affiliation:
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Laboratoire de Pharmacologie Cellulaire de I'E.P.H.E., Institut Biomédical des Cordeliers, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage, Articular / cytology*, drug effects Cell Division / drug effects Cells, Cultured Flow Cytometry Free Radicals Oxygen / pharmacology* Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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