Document Detail


Effects of oxidative stress on prion protein expression in PC12 cells.
MedLine Citation:
PMID:  9641527     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PC12 cells are known to express the prion protein, a normal cell surface glycoprotein. This protein is upregulated in PC12 cells differentiated with nerve growth factor. A neurotoxic prion protein peptide, PrP106-126, is not toxic to PC12 cells alone. PrP106-126 is toxic to PC12 cells co-cultured with microglia and more so to NGF-differentiated PC12 cells. PC12 cells selected for resistance to either copper toxicity or oxidative stress have higher levels of PrP(C) expression. Both PC12 variants are more sensitive to the toxicity of PrP106-126. This suggests that PC12 sensitivity to PrP106-126 toxicity is related to prion protein expression and not to a state of high differentiation induced by NGF. Variants of PC12 cells that are more resistant to copper toxicity have higher levels of anti-oxidant enzymes, superoxide dismutase and glutathione peroxidase. Our results suggest that cells expressing higher levels of PrP(C) have higher resistance to oxidative stress or copper toxicity but are more sensitive to PrP106-126 toxicity. Prion protein expression may be involved in both the metabolism of copper and resistance to oxidative stress. Increased cellular resistance to copper toxicity may be partly related to increased activity of anti-oxidant enzymes.
Authors:
D R Brown; B Schmidt; H A Kretzschmar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience     Volume:  15     ISSN:  0736-5748     ISO Abbreviation:  Int. J. Dev. Neurosci.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-08-20     Completed Date:  1998-08-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8401784     Medline TA:  Int J Dev Neurosci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  961-72     Citation Subset:  IM    
Affiliation:
Institut für Neuropathologie, Universität Göttingen, Germany. drb33@cam.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Glutathione Peroxidase / metabolism
Oxidative Stress / physiology*
PC12 Cells / drug effects,  metabolism*
Peptide Fragments / poisoning
Prions / metabolism*,  poisoning
Rats
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Peptide Fragments; 0/Prions; 0/prion protein (106-126); EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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