Document Detail

Effects of oxidative stress on glycerolipid acyl turnover in rat hepatocytes.
MedLine Citation:
PMID:  9307931     Owner:  NLM     Status:  MEDLINE    
Lipid peroxidation was induced in freshly isolated rat hepatocytes by incubation in the presence of Fe3+, resulting in accumulation of thiobarbituric acid reactive substances. Analysis of lipid classes revealed that the levels and fatty acid compositions of the two major phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), remained unchanged but the levels of triacylglycerols (TAG) were significantly reduced, and some of their polyunsaturated fatty acids were selectively lost as the result of oxidant treatment. Acyl turnover in PC and PE as determined by 18O incorporation from H2 (18)O-containing media remained largely unchanged during oxidant treatment, while some increased turnover of the saturated fatty acids in TAG was observed. We hypothesize that constitutive recycling of membrane phospholipids rather than selective in situ repair eliminates peroxidized species of PC and PE. TAG could serve as an expendable fatty acid reserve, providing a limited but very dynamic pool of polyunsaturated fatty acids for the resynthesis of phospholipids.
J Girón-Calle; P C Schmid; H H Schmid
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  32     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-01-06     Completed Date:  1998-01-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  917-23     Citation Subset:  IM    
Hormel Institute, University of Minnesota, Austin 55912, USA.
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MeSH Terms
Cells, Cultured
Fatty Acids / metabolism
Fatty Acids, Unsaturated / metabolism
Ferric Compounds / metabolism
Glycerophosphates / metabolism*
Lipid Metabolism*
Lipid Peroxidation
Lipid Peroxides / metabolism
Liver / metabolism*
Membrane Lipids / metabolism
Oxidative Stress*
Oxygen Isotopes
Phosphatidylcholines / metabolism
Phosphatidylethanolamines / metabolism
Rats, Sprague-Dawley
Thiobarbituric Acid Reactive Substances / metabolism
Triglycerides / metabolism
Grant Support
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Ferric Compounds; 0/Glycerophosphates; 0/Lipid Peroxides; 0/Membrane Lipids; 0/Oxygen Isotopes; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Thiobarbituric Acid Reactive Substances; 0/Triglycerides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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