Document Detail

Effects of oxidation on the hydrolysis by cholesterol esterase of sitosteryl esters as compared to a cholesteryl ester.
MedLine Citation:
PMID:  19464305     Owner:  NLM     Status:  MEDLINE    
Phytosteryl esters (PE) are used as ingredients in functional food to decrease plasma concentration of low density lipoprotein-cholesterol (LDL-C). Effective impairment of cholesterol absorption by PE suggests that these esters are hydrolyzed by the pancreatic cholesterol esterase (CEase, EC and the liberated sterol may interfere with cholesterol reducing its intestinal absorption. PE-enriched foods are marketed for cooking purposes, and temperature is one of the most important factors leading to the formation of oxidation products. Very little is known about the outcome of PE oxides during the digestive process. A new analytical method based on mass spectrometric detection directly after enzymatic reaction was developed to determine in vitro the activity of CEase on PE and their oxides present in functional food. Using this method, we identified a new inhibitor of CEase: sitosteryl 9,10-dihydroxystearate, which behaves as a non-competitive inhibitor of the hydrolysis of cholesteryl oleate and sitosteryl oleate.
Diane Julien-David; Saïd Ennahar; Michel Miesch; Philippe Geoffroy; Francis Raul; Dalal Aoude-Werner; Jean-Marc Lessinger; Eric Marchioni
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-21
Journal Detail:
Title:  Steroids     Volume:  74     ISSN:  1878-5867     ISO Abbreviation:  Steroids     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  832-6     Citation Subset:  IM    
IPHC, UMR 7178, Laboratoire de Chimie Analytique et Sciences de l'Aliment-Université de Strasbourg, Faculté de Pharmacie, 74 route de Rhin, 67640 Illkirch-Graffenstaden, France.
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MeSH Terms
Antilipemic Agents / chemistry*,  metabolism*
Cholesterol Esters / metabolism*
Esters / chemistry*
Hydrolysis / drug effects
Oleic Acid / chemistry,  metabolism
Oxides / chemistry,  metabolism
Sitosterols / chemistry*,  metabolism*
Stearates / chemistry,  pharmacology
Sterol Esterase / antagonists & inhibitors,  metabolism*
Reg. No./Substance:
0/Antilipemic Agents; 0/Cholesterol Esters; 0/Esters; 0/Oxides; 0/Sitosterols; 0/Stearates; 112-80-1/Oleic Acid; 5779-62-4/sitosterol; EC Esterase

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