Document Detail


Effects of ovarian theca cells on granulosa cell differentiation during gonadotropin-independent follicular growth in cattle.
MedLine Citation:
PMID:  16541462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effects of theca cells or FSH on granulosa cell differentiation and steroid production during bovine early follicular growth, using a co-culture system in which granulosa and theca cells were cultured on opposite sides of a collagen membrane. Follicular cells were isolated from early antral follicles (2-4 mm) that were assumed to be in gonadotropin-independent phase and just before recruitment into a follicular wave. Granulosa cells were cultured under serum-free conditions with and without theca cells or recombinant human FSH to test their effects on granulosa cell differentiation. Messenger RNA levels for P450 aromatase (aromatase), P450 cholesterol side chain cleavage (P450scc), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), LH receptor (LHr), and steroidogenic acute regulatory protein (StAR) in granulosa cells were measured by real-time quantitative RT-PCR analysis. FSH enhanced aromatase mRNA expression in granulosa cells, but did not alter estradiol production. FSH also enhanced mRNA expression for P450scc, LHr, and StAR in granulosa cells, resulting in an increase in progesterone production. In contrast, theca cells enhanced aromatase mRNA expression in granulosa cells resulting in an increase in estradiol production. Theca cells did not alter progesterone production and mRNA expression in granulosa cells for P450scc, 3beta-HSD, LHr, and StAR. The results of the present study indicate that theca cells are involved in both rate-limiting steps in estrogen production, i.e., androgen substrate production and aromatase regulation, and that theca cell-derived factors regulate estradiol and progesterone production in a way that reflects steroidogenesis during the follicular phase of the estrous cycle.
Authors:
Makoto Orisaka; Tetsuya Mizutani; Kimihisa Tajima; Sanae Orisaka; Ken-ichi Shukunami; Kaoru Miyamoto; Fumikazu Kotsuji
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  73     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-03-29     Completed Date:  2007-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  737-44     Citation Subset:  IM    
Copyright Information:
Copyright 2006 Wiley-Liss, Inc.
Affiliation:
Department of Obstetrics and Gynecology, University of Fukui, Fukui, Japan.
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxysteroid Dehydrogenases / genetics,  metabolism
Animals
Aromatase / genetics,  metabolism
Cattle
Cell Differentiation / physiology*
Cell Proliferation
Cells, Cultured
Cholesterol Side-Chain Cleavage Enzyme / genetics,  metabolism
Coculture Techniques
Culture Media, Serum-Free
Cytochrome P-450 Enzyme System
Female
Follicle Stimulating Hormone / genetics,  metabolism
Gonadotropins / genetics,  metabolism*
Granulosa Cells / cytology,  physiology*
Humans
Ovarian Follicle / cytology*,  growth & development*,  metabolism
Phosphoproteins / genetics,  metabolism
Receptors, LH / genetics,  metabolism
Theca Cells / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Gonadotropins; 0/Phosphoproteins; 0/Receptors, LH; 0/steroidogenic acute regulatory protein; 9002-68-0/Follicle Stimulating Hormone; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.14.1/Aromatase; EC 1.14.15.6/Cholesterol Side-Chain Cleavage Enzyme

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