Document Detail


Effects of ornithine on neutrophil (PMN) free amino acid and alpha-keto acid profiles and immune functions in vitro.
MedLine Citation:
PMID:  15538644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to determine the effects of ornithine on polymorphonuclear leucocyte (PMN) free amino- and alpha-keto acid profiles, superoxide anion (O2-) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase activity (MPO). Exogenous ornithine significantly increased PMN asparagine, glutamine, aspartate, glutamate, arginine, citrulline, alanine, alpha-ketoglutarate and pyruvate as intracellular ornithine increased. Concerning PMN immune function markers ornithine increased H2O2-generation and MPO activity while O2- -formation was decreased. We believe therefore that ornithine is important for affecting PMN "susceptible free amino- and alpha-keto acid pool" although the mechanisms are not yet clear. This may be one of the determinants in PMN nutrition considerably influencing and modulating PMN host defense capability.
Authors:
J Mühling; M Fuchs; M Campos; J Gonter; A Sablotzki; J Engel; I D Welters; M Wolff; R Matejec; M G Dehne; T Menges; M Krüll; G Hempelmann
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Publication Detail:
Type:  Journal Article     Date:  2004-11-05
Journal Detail:
Title:  Amino acids     Volume:  27     ISSN:  0939-4451     ISO Abbreviation:  Amino Acids     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  313-9     Citation Subset:  IM    
Affiliation:
Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig University, Giessen, Germany. joerg.muehling@chiru.med.uni-giessen.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Amino Acids / blood*
Cells, Cultured
Dose-Response Relationship, Drug
Humans
Hydrogen Peroxide / blood
Keto Acids / blood*
Male
Neutrophils / drug effects,  immunology*,  metabolism*
Ornithine / blood,  pharmacology*
Oxidation-Reduction
Peroxidase / blood,  drug effects
Superoxides / blood
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Keto Acids; 11062-77-4/Superoxides; 7006-33-9/Ornithine; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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