| Effects of opsonization of Rhodococcus equi on bacterial viability and phagocyte activation. | |
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MedLine Citation:
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PMID: 22023124 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective-To investigate the effect of opsonization of Rhodococcus equi with R equi-specific antibodies in plasma on bacterial viability and phagocyte activation in a cell culture model of infection. Sample-Neutrophils and monocyte-derived macrophages from 6 healthy 1-week-old foals and 1 adult horse. Procedures-Foal and adult horse phagocytes were incubated with either opsonized or nonopsonized bacteria. Opsonization was achieved by use of plasma containing high or low concentrations of R equi-specific antibodies. Phagocyte oxidative burst activity was measured by use of flow cytometry, and macrophage tumor necrosis factor (TNF)-α production was measured via an ELISA. Extracellular and intracellular bacterial viability was measured with a novel R equi-luciferase construct that used a luminometer. Results-Opsonized bacteria increased oxidative burst activity in adult horse phagocytes, and neutrophil activity was dependent on the concentration of specific antibody. Secretion of TNF-α was higher in macrophages infected with opsonized bacteria. Opsonization had no significant effect on bacterial viability in macrophages; however, extracellular bacterial viability was decreased in broth containing plasma with R equi-specific antibodies, compared with viability in broth alone. Conclusions and Clinical Relevance-The use of plasma enriched with specific antibodies for the opsonization of R equi increased the activation of phagocytes and decreased bacterial viability in the extracellular space. Although opsonized R equi increased TNF-α secretion and oxidative burst in macrophages, additional factors may be necessary for effective intracellular bacterial killing. These data have suggested a possible role of plasma antibody in protection of foals from R equi pneumonia. |
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Authors:
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Dominic R Dawson; Daryl V Nydam; Christopher T Price; James E Graham; Michael H Cynamon; Thomas J Divers; Maria Julia B Felippe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of veterinary research Volume: 72 ISSN: 1943-5681 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-10-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: United States |
Other Details:
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Languages: eng Pagination: 1465-75 Citation Subset: IM |
Affiliation:
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Departments of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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