| Effects of the omega-6:omega-3 fatty acid ratio of fat emulsions on the fatty acid composition in cell membranes and the anti-inflammatory action. | |
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MedLine Citation:
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PMID: 20467008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: This study investigated the effects of parenterally administered fish oil (FO) on the fatty acid composition in rats to determine the optimal omega-6:omega-3 polyunsaturated fatty acid (PUFA) ratio of fat emulsions to achieve an anti-inflammatory effect. METHODS: Male Sprague-Dawley rats were infused a parenteral nutrition (PN) solution containing fat emulsions with different omega-6:omega-3 PUFA ratios. The fatty acid content of phospholipids in the membranes of splenocytes was analyzed by gas chromatography (experiment 1). In addition, the amounts of leukotriene (LT) B(4) and LTB(5) released from peritoneal polymorphonuclear leukocytes (PMNs) were measured by high-performance liquid chromatography (experiment 2). RESULTS: In experiment 1, after infusion of the fat emulsion containing FO, the omega-3 PUFA content in cell membranes rose to 70% of the peak value on day 1 and nearly reached a plateau on day 3. The highest ratio of eicosapentaenoic acid (EPA) to arachidonic acid (AA) was achieved by administering a PN solution with the smallest omega-6:omega-3 PUFA ratio. In experiment 2, a larger amount of LTB(5) was released from Ca-ionophore-stimulated PMNs taken from rats given a larger quantity of FO. The ratio of LTB(5):LTB(4) released from PMNs correlated positively with the EPA:AA ratio in the membranous phospholipid and in serum. CONCLUSIONS: The omega-3 PUFAs were readily incorporated into the cell membrane within 3 days of infusion with the fat emulsion. The EPA:AA ratio in membranous phospholipid in PMNs was positively correlated with the LTB(5):LTB(4) production ratio and was a good indicator of anti-inflammatory effects. |
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Authors:
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Akifumi Hagi; Mitsuo Nakayama; Wataru Shinzaki; Seiji Haji; Harumasa Ohyanagi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: JPEN. Journal of parenteral and enteral nutrition Volume: 34 ISSN: 0148-6071 ISO Abbreviation: JPEN J Parenter Enteral Nutr Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-05-14 Completed Date: 2010-10-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804134 Medline TA: JPEN J Parenter Enteral Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 263-70 Citation Subset: IM |
Affiliation:
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R&D Center, Otsuka Pharmaceutical Factory, Inc, Tokushima, Japan. hagiak@otsukakj.co.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents / pharmacology*, therapeutic use Arachidonic Acid / analysis Calcium / metabolism Cell Membrane / drug effects*, metabolism Eicosapentaenoic Acid / analogs & derivatives, analysis, metabolism Fat Emulsions, Intravenous / chemistry, pharmacology*, therapeutic use Fatty Acids, Omega-3 / metabolism, pharmacology*, therapeutic use Fatty Acids, Omega-6 / metabolism, pharmacology*, therapeutic use Inflammation / drug therapy Leukotriene B4 / analogs & derivatives, metabolism Leukotrienes / metabolism* Lipid Metabolism* Male Neutrophils / metabolism Parenteral Nutrition Phospholipids / chemistry Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Fat Emulsions, Intravenous; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/Leukotrienes; 0/Phospholipids; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid; 71160-24-2/Leukotriene B4; 7440-70-2/Calcium; 80445-66-5/leukotriene B5 |
| Comments/Corrections | |
Comment In:
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JPEN J Parenter Enteral Nutr. 2010 May-Jun;34(3):344-5
[PMID:
19884351
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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