Document Detail


Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase.
MedLine Citation:
PMID:  19723076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidative stress plays a critical role in apoptosis of gastric epithelial cells. Omega-3 fatty acids show anti-inflammatory and/or anticancer effects and regulate apoptosis in various cells. In this study, we aimed to investigate whether omega-3 fatty acids inhibit oxidative stress-induced apoptosis of gastric epithelial cells. The cells received oxidative stress caused by silica-immobilized glucose oxidase acting on beta-D-glucose and cultured in the absence or presence of alpha-linolenic acid or docosahexanoic acid. Viable cell numbers, levels of H(2)O(2) in the medium, DNA fragmentation, and protein levels of p53 and Bax were determined. As a result, silica-immobilized glucose oxidase acting on beta-D-glucose consistently and reproducibly produced H(2)O(2), which decreased cell viability and increased DNA fragmentation of the cells. Omega-3 fatty acids inhibited oxidative stress-induced cell death, DNA fragmentation, and induction of p53 and Bax of the cells. The silica-immobilized glucose oxidase could be a useful tool for studies on oxidative stress-induced cellular events because it is reusable and forms a stable enzyme system acting on glucose. Omega-3 fatty acids may be beneficial for preventing oxidative stress-induced apoptosis by inhibiting apoptotic gene expression and DNA fragmentation of gastric epithelial cells.
Authors:
Ji Hoon Yu; Sin-Gun Kang; Un-Young Jung; Chul-Ho Jun; Hyeyoung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1171     ISSN:  1749-6632     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  359-64     Citation Subset:  IM    
Affiliation:
Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism,  pathology
Apoptosis / drug effects*
Blotting, Western
Cell Line, Tumor
Cell Survival / drug effects
DNA Fragmentation / drug effects
Docosahexaenoic Acids / pharmacology
Enzyme-Linked Immunosorbent Assay
Enzymes, Immobilized / metabolism*
Fatty Acids, Omega-3 / pharmacology*
Glucose / pharmacology
Glucose Oxidase / metabolism*
Humans
Hydrogen Peroxide / metabolism
Silicon Dioxide / chemistry
Stomach Neoplasms / metabolism,  pathology
Tumor Suppressor Protein p53 / metabolism
alpha-Linolenic Acid / pharmacology
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Fatty Acids, Omega-3; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 25167-62-8/Docosahexaenoic Acids; 463-40-1/alpha-Linolenic Acid; 50-99-7/Glucose; 7631-86-9/Silicon Dioxide; 7722-84-1/Hydrogen Peroxide; EC 1.1.3.4/Glucose Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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