| Effects of omega-3 fatty acids on components of the transforming growth factor beta-1 pathway: implication for dietary modification and prevention in ovarian cancer. | |
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MedLine Citation:
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PMID: 19268879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: We previously demonstrated that omega-3 fatty acids (OM-3FAs) have definitive inhibitory effects on ovarian cancer cell lines. We sought to determine whether the inhibitory effects of OM-3FAs were mediated by the transforming growth factor (TGF)-beta1 signaling pathway. STUDY DESIGN: Ovarian cancer cell lines were grown at 37 degrees C in 5% CO(2) and treated with OM-3FAs, omega-6 fatty acids, and control at different concentrations for 24-72 hours. Enzyme-linked immunosorbent assay (ELISA) assay and Western blot analysis were used to measure TGF-beta1, phosphorylated mothers against decapentaplegic (Smad)-3 and p21 protein levels. RESULTS: An ELISA assay demonstrated that OM-3FA treatment increased TGF-beta1 in all 3 Hey cell lines (P < .05). In both SKOV-3 and OVCAR-3 cells, TGF-beta1 levels were not significantly increased. Western blots confirmed increases in TGF-beta1, Smad-3 and p21 protein levels in Hey and HeyC2 but not SKOV-3 and OVCAR-3 cells. CONCLUSION: OM-3FAs increased the level of TGF-beta1, Smad-3, and p21 protein in ovarian cancer cells known to be more sensitive to their inhibitory effect. |
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Authors:
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Arun Sharma; Jennifer Belna; Joseph Espat; Gustavo Rodriguez; Vernon T Cannon; Jean A Hurteau |
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Publication Detail:
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Type: Journal Article Date: 2009-03-09 |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 200 ISSN: 1097-6868 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 516.e1-6 Citation Subset: AIM; IM |
Affiliation:
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Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Illinois Medical Center at Chicago, Chicago, IL, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzamides Cell Line, Tumor Cyclin-Dependent Kinase Inhibitor p21 / metabolism Fatty Acids, Omega-3 / pharmacology* Fatty Acids, Omega-6 / pharmacology Female Humans Ovarian Neoplasms / diet therapy, metabolism*, prevention & control* Phosphorylation / drug effects Signal Transduction / drug effects* Smad3 Protein / metabolism Transforming Growth Factor beta1 / metabolism* Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Benzamides; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/SMAD3 protein, human; 0/Smad3 Protein; 0/Transforming Growth Factor beta1; 0/Tumor Suppressor Protein p53; 82558-50-7/isoxaben |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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