Document Detail


Effects of nitric oxide on human spermatozoa activity, fertilization and mouse embryonic development.
MedLine Citation:
PMID:  15204684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was conducted to investigate the effects of nitric oxide (NO) on human sperm activity, human sperm-oocyte fusion and mouse embryonic development. Results showed that various concentrations of NO synthase blocker, N(omega)-nitro-L-arginine methyl ester, did not affect sperm cell motility at 0, 1, 2 or 4 hr, respectively. In contrast, sodium nitroprusside (SNP) significantly inhibited sperm cell motility and caused apoptosis. The adversely dose-dependent effect was only observed if SNP was freshly prepared. Adenosine triphosphate reversed the hazardous effect of SNP on sperm activity/viability. Hemoglobin neutralized the adverse effect of SNP. In hemi-zona sperm fusion test, the number of sperm bound to the zona in the presence of 10(-4) M SNP was significantly less than the control group. SNP at 10(-4) M caused all mouse embryonic development arrest. 46% and 56% of zygote reached the blastocyst stage with the treatment of 10(-6) M and 10(-8) M SNP, respectively, while the control reached 70%. NO adversely affected human sperm activity, human sperm-zona binding and embryonic development. It would appear that high concentration of NO may potentially decrease fertility.
Authors:
T-P Wu; B-M Huang; H-C Tsai; M-C Lui; M-Y Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of andrology     Volume:  50     ISSN:  0148-5016     ISO Abbreviation:  Arch. Androl.     Publication Date:    2004 May-Jun
Date Detail:
Created Date:  2004-06-18     Completed Date:  2004-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806755     Medline TA:  Arch Androl     Country:  England    
Other Details:
Languages:  eng     Pagination:  173-9     Citation Subset:  IM    
Affiliation:
Department of Gynecology and Obstetrics, Sin-Lau Christian Hospital, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology
Animals
Apoptosis / drug effects
Embryonic Development / drug effects*
Fertilization / drug effects*
Humans
Male
Mice
Nitric Oxide / metabolism*
Nitroprusside / pharmacology
Sperm Motility / drug effects
Spermatozoa / metabolism*
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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