| Effects of nitric oxide on human spermatozoa activity, fertilization and mouse embryonic development. | |
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MedLine Citation:
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PMID: 15204684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was conducted to investigate the effects of nitric oxide (NO) on human sperm activity, human sperm-oocyte fusion and mouse embryonic development. Results showed that various concentrations of NO synthase blocker, N(omega)-nitro-L-arginine methyl ester, did not affect sperm cell motility at 0, 1, 2 or 4 hr, respectively. In contrast, sodium nitroprusside (SNP) significantly inhibited sperm cell motility and caused apoptosis. The adversely dose-dependent effect was only observed if SNP was freshly prepared. Adenosine triphosphate reversed the hazardous effect of SNP on sperm activity/viability. Hemoglobin neutralized the adverse effect of SNP. In hemi-zona sperm fusion test, the number of sperm bound to the zona in the presence of 10(-4) M SNP was significantly less than the control group. SNP at 10(-4) M caused all mouse embryonic development arrest. 46% and 56% of zygote reached the blastocyst stage with the treatment of 10(-6) M and 10(-8) M SNP, respectively, while the control reached 70%. NO adversely affected human sperm activity, human sperm-zona binding and embryonic development. It would appear that high concentration of NO may potentially decrease fertility. |
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Authors:
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T-P Wu; B-M Huang; H-C Tsai; M-C Lui; M-Y Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of andrology Volume: 50 ISSN: 0148-5016 ISO Abbreviation: Arch. Androl. Publication Date: 2004 May-Jun |
Date Detail:
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Created Date: 2004-06-18 Completed Date: 2004-11-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7806755 Medline TA: Arch Androl Country: England |
Other Details:
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Languages: eng Pagination: 173-9 Citation Subset: IM |
Affiliation:
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Department of Gynecology and Obstetrics, Sin-Lau Christian Hospital, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology Animals Apoptosis / drug effects Embryonic Development / drug effects* Fertilization / drug effects* Humans Male Mice Nitric Oxide / metabolism* Nitroprusside / pharmacology Sperm Motility / drug effects Spermatozoa / metabolism* |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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