Document Detail

Effects of a nitric oxide donor on and correlation of changes in cyclic nucleotide levels with experimental vasospasm.
MedLine Citation:
PMID:  12590692     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Vasospasm after subarachnoid hemorrhage (SAH) may result from hemoglobin-mediated removal of nitric oxide (NO) from the arterial wall. We tested the ability of the long-acting, water-soluble, NO donor (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-1,2-diolate (DETA/NO), delivered via continuous intracisternal infusion, to prevent vasospasm in a nonhuman primate model of SAH. METHODS: First, vasorelaxation in response to DETA/NO was characterized in vitro by using monkey basilar artery rings under isometric tension. Next, monkeys were randomized to undergo angiography, unilateral SAH, and no treatment (SAH only, n = 4) or treatment with DETA/NO (1 mmol/L, 12 ml/d, n = 4) or decomposed DETA/NO (at the same dose, n = 4). Vasospasm was assessed by angiography, which was performed on Day 0 and Day 7. Levels of cyclic adenosine monophosphate and cyclic guanosine monophosphate (cGMP) were measured in cerebral arteries on Day 7. RESULTS: DETA/NO produced significant relaxation of monkey arteries in vitro, which reached a maximum at concentrations of 10(-5) mol/L. In monkeys, angiography demonstrated significant vasospasm of the right intradural cerebral arteries in all three groups, with no significant difference in vasospasm among the groups (P > 0.05, analysis of variance). The ratios of cGMP or cyclic adenosine monophosphate levels in the right and left middle cerebral arteries were not different among the groups (P > 0.05, analysis of variance). There was no significant correlation between arterial cGMP contents and the severity of vasospasm. CONCLUSION: DETA/NO did not prevent vasospasm. There was no correlation between the severity of vasospasm and cyclic adenosine monophosphate and cGMP levels in the cerebral arteries. These results suggest that events downstream of cyclic nucleotides may be abnormal during vasospasm.
Yasuo Aihara; Babak S Jahromi; Reza Yassari; Tetsuro Sayama; R Loch Macdonald
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurosurgery     Volume:  52     ISSN:  0148-396X     ISO Abbreviation:  Neurosurgery     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-02-19     Completed Date:  2003-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802914     Medline TA:  Neurosurgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  661-7; discussion 666-7     Citation Subset:  IM    
Department of Surgery, Pritzker School of Medicine and University of Chicago Medical Center, Chicago, Illinois 60637, USA.
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MeSH Terms
Cerebral Angiography
Cyclic AMP / blood*
Cyclic GMP / blood*
Disease Models, Animal
Macaca fascicularis
Middle Cerebral Artery / drug effects,  radiography
Nitric Oxide Donors / therapeutic use*
Severity of Illness Index
Subarachnoid Hemorrhage / blood*,  complications*,  radiography
Triazenes / therapeutic use*
Vasospasm, Intracranial / blood*,  etiology,  prevention & control*
Reg. No./Substance:
0/1-hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene; 0/Nitric Oxide Donors; 0/Triazenes; 60-92-4/Cyclic AMP; 7665-99-8/Cyclic GMP

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