| Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia. | |
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MedLine Citation:
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PMID: 20131324 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Maternal smoking during pregnancy increases the incidence and severity of respiratory infections in neonates. Surfactant proteins A and D (SP-A and SP-D, respectively) are components of pulmonary innate immunity and have an important role in defense against inhaled pathogens. The purpose of this study was to determine if nicotine exposure during the third trimester of pregnancy alters the expression of SP-A and SP-D of fetal lung epithelia. Pregnant ewes were assigned to four groups; a nicotine-exposed full-term and pre-term group, and control full-term and pre-term group. Lung tissue was collected for Western blot and IHC analysis of SP-A level, Western blot analysis of SP-D level and qPCR analysis of SP-A and SP-D mRNA expression. Exposure to nicotine significantly decreased SP-A gene expression (P = 0.01) and SP-A protein level in pre-term lambs. This finding suggests that maternal nicotine exposure during the last trimester of pregnancy alters a key component of lung innate immunity in offspring. |
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Authors:
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Tatjana Lazic; Milan Matic; Jack M Gallup; Albert Van Geelen; David K Meyerholz; Branka Grubor; Paula M Imerman; Marcia M M A de-Macedo; Mark R Ackermann |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Pediatric pulmonology Volume: 45 ISSN: 1099-0496 ISO Abbreviation: Pediatr. Pulmonol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-26 Completed Date: 2010-06-16 Revised Date: 2011-08-30 |
Medline Journal Info:
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Nlm Unique ID: 8510590 Medline TA: Pediatr Pulmonol Country: United States |
Other Details:
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Languages: eng Pagination: 255-62 Citation Subset: IM |
Affiliation:
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Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA. tlazic@iastate.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Cutaneous Animals Blotting, Western Cotinine / blood Disease Models, Animal Female Fetal Organ Maturity / drug effects* Immunohistochemistry Lung / embryology* Nicotine / administration & dosage, adverse effects* Pregnancy Pregnancy Trimester, Third Pulmonary Surfactant-Associated Protein A / genetics, metabolism* Pulmonary Surfactant-Associated Protein D / genetics, metabolism* RNA, Messenger / metabolism Sheep Smoking / adverse effects |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI062787-05/AI/NIAID NIH HHS; R01AI062787/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Pulmonary Surfactant-Associated Protein A; 0/Pulmonary Surfactant-Associated Protein D; 0/RNA, Messenger; 486-56-6/Cotinine; 54-11-5/Nicotine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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