Document Detail

Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia.
MedLine Citation:
PMID:  20131324     Owner:  NLM     Status:  MEDLINE    
Maternal smoking during pregnancy increases the incidence and severity of respiratory infections in neonates. Surfactant proteins A and D (SP-A and SP-D, respectively) are components of pulmonary innate immunity and have an important role in defense against inhaled pathogens. The purpose of this study was to determine if nicotine exposure during the third trimester of pregnancy alters the expression of SP-A and SP-D of fetal lung epithelia. Pregnant ewes were assigned to four groups; a nicotine-exposed full-term and pre-term group, and control full-term and pre-term group. Lung tissue was collected for Western blot and IHC analysis of SP-A level, Western blot analysis of SP-D level and qPCR analysis of SP-A and SP-D mRNA expression. Exposure to nicotine significantly decreased SP-A gene expression (P = 0.01) and SP-A protein level in pre-term lambs. This finding suggests that maternal nicotine exposure during the last trimester of pregnancy alters a key component of lung innate immunity in offspring.
Tatjana Lazic; Milan Matic; Jack M Gallup; Albert Van Geelen; David K Meyerholz; Branka Grubor; Paula M Imerman; Marcia M M A de-Macedo; Mark R Ackermann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Pediatric pulmonology     Volume:  45     ISSN:  1099-0496     ISO Abbreviation:  Pediatr. Pulmonol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-26     Completed Date:  2010-06-16     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  8510590     Medline TA:  Pediatr Pulmonol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  255-62     Citation Subset:  IM    
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MeSH Terms
Administration, Cutaneous
Blotting, Western
Cotinine / blood
Disease Models, Animal
Fetal Organ Maturity / drug effects*
Lung / embryology*
Nicotine / administration & dosage,  adverse effects*
Pregnancy Trimester, Third
Pulmonary Surfactant-Associated Protein A / genetics,  metabolism*
Pulmonary Surfactant-Associated Protein D / genetics,  metabolism*
RNA, Messenger / metabolism
Smoking / adverse effects
Grant Support
Reg. No./Substance:
0/Pulmonary Surfactant-Associated Protein A; 0/Pulmonary Surfactant-Associated Protein D; 0/RNA, Messenger; 54-11-5/Nicotine; K5161X06LL/Cotinine

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