| Effects of neonatal exposure to diethylstilbestrol on protein expression by vagina and uterus in mice. | |
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MedLine Citation:
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PMID: 1378305 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neonatal treatment of female mice with diethylstilbestrol (DES) results in genital tract abnormalities including ovary-independent vaginal proliferation and cornification. Protein profiles were examined in vagina and uterus from 45-day-old, ovariectomized C57BL/Tw mice which had been given 5 daily injections of 2 micrograms DES or oil vehicle alone from the day of birth, and in those from 45-day-old, ovariectomized mice given 3 daily injections of 0.1 microgram DES from 42 days of age. Proteins extracted were analyzed by two-dimensional polyacrylamide gel electrophoresis. In the vagina, expression of 37 non-keratin proteins was altered by postpubertal injections of DES as compared with the controls. In the neonatally DES-exposed vagina, expression of 26 of 37 proteins was altered as compared with controls. In the uterus, expression of 22 proteins was altered in the postpubertally DES-exposed group as compared with that in the control; however, the protein expression pattern of the neonatally DES-exposed group closely resembled that of the control except for one protein (no. 23, pI = 6.1, MW = 39.5 kDa) which was specifically increased in the neonatally DES-exposed group. By immunoblot analysis, 6 keratin polypeptides (49.5, 50, 52, 53, 57 and 58 kDa) were identified in vaginae of ovariectomized mice exposed neonatally and postpubertally to DES and of the controls. These results indicate that neonatal DES exposure induces organ specific alterations in the synthesis of proteins in mouse vagina and uterus. |
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Authors:
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Y Takamatsu; T Iguchi; N Takasugi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: In vivo (Athens, Greece) Volume: 6 ISSN: 0258-851X ISO Abbreviation: In Vivo Publication Date: 1992 Jan-Feb |
Date Detail:
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Created Date: 1992-08-20 Completed Date: 1992-08-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8806809 Medline TA: In Vivo Country: GREECE |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Affiliation:
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Graduate School of Integrated Science, Yokohama City University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn* Diethylstilbestrol / pharmacology*, toxicity Electrophoresis, Gel, Two-Dimensional Epithelium / pathology Female Gene Expression Regulation / drug effects* Keratins / biosynthesis Mice Mice, Inbred C57BL Organ Specificity Ovariectomy Protein Biosynthesis* Sexual Maturation Uterus / growth & development, metabolism*, pathology Vagina / growth & development, metabolism*, pathology Vaginal Diseases / chemically induced*, metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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56-53-1/Diethylstilbestrol; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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