Document Detail

Effects of neonatal diethylstilbestrol (DES) exposure on morphology and growth patterns of endometrial epithelial cells in CD-1 mice.
MedLine Citation:
PMID:  10356709     Owner:  NLM     Status:  MEDLINE    
The effects of neonatal diethylstilbestrol (DES) exposure on the morphology and proliferating patterns of endometrial epithelial cells were investigated at various stages of development in mice. Female CD-1 mice were given daily subcutaneous injections of 2 micrograms of DES in corn oil or corn oil alone (control) at 1-5 days of age and were killed at 5, 6, 7, 8, 15, and 22 days of age. At 5 days of age, the uteri of DES-treated mice had expanded lumina and undulated luminal surfaces lined by slightly elongated epithelial cells. At 6-8 days of age, marked infolding of clusters of hypertrophic elongated luminal epithelial cells was present; uteri had disorganized endometrial stromal and myometrial layers. At 15 and 22 days of age, the tissues from DES-treated mice had decreased numbers of endometrial glands, minimal stromal fibrosis, and smaller uterine horns than did the controls. Ultrastructurally, the endometrial epithelial cells of DES-treated mice at 5 and 8 days of age had distorted nuclei with condensed matrix and abundant secretory granules associated with rough endoplasmic reticulum and Golgi apparatus. At 8 days of age, an accumulation of fingerlike cytoplasmic processes that extended into the separated intercellular spaces and along the basal aspects of the endometrial epithelial cells were also observed. At 5-8 days of age, the proliferative activity of endometrial epithelial cells in DES-treated mice, identified by bromodeoxyuridine labeling, was significantly lower (10.5-1.7%) than that of the controls (25.5-19.8%). In situ analysis of endometrial luminal epithelial cells for DNA fragmentation representing apoptosis revealed < or = 0.1% and > 10% in the DES-treated and control mice at 5-8 days of age, respectively. The data show that cell cycle kinetics, in addition to changes in morphology, are altered in the developing mouse uterus following neonatal exposure to DES.
A Yoshida; R R Newbold; D Dixon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicologic pathology     Volume:  27     ISSN:  0192-6233     ISO Abbreviation:  Toxicol Pathol     Publication Date:    1999 May-Jun
Date Detail:
Created Date:  1999-07-16     Completed Date:  1999-07-16     Revised Date:  2009-07-01    
Medline Journal Info:
Nlm Unique ID:  7905907     Medline TA:  Toxicol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  325-33     Citation Subset:  IM    
Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
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MeSH Terms
Animals, Newborn
Apoptosis / drug effects
Bromodeoxyuridine / metabolism
Cell Count
Cell Division / drug effects
Cytoplasm / ultrastructure
Diethylstilbestrol / toxicity*
Endometrium / drug effects*,  growth & development,  metabolism,  pathology
Epithelial Cells / drug effects,  metabolism,  pathology
Estrogens, Non-Steroidal / toxicity*
In Situ Nick-End Labeling
Injections, Subcutaneous
Microscopy, Electron
Stromal Cells / drug effects,  metabolism,  pathology
Reg. No./Substance:
0/Estrogens, Non-Steroidal; 56-53-1/Diethylstilbestrol; 59-14-3/Bromodeoxyuridine

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