Document Detail


Effects of natural tachykinins on ovine lower urinary tract smooth muscle.
MedLine Citation:
PMID:  11679016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Numerous studies have demonstrated that the urinary bladder is particularly sensitive to tachykinins; rat, rabbit and guinea pig bladders, besides human detrusor, have been the most extensively studied, whereas very little is known about most large animal detrusors. The aim of this work was to study natural tachykinin activity on the lower urinary tract of ovine to make a comparison with data obtained in laboratory animals. 2. As in other animal species, tachykinins are also able to contract ovine bladder smooth muscle. 3. The results reported in this study indicate that in ovine bladder, neurokinin 2 (NK2) receptors are expressed most. In fact, on lamb and sheep bladder neurokinin A (NKA), a NK2- almost selective peptide, was shown to be > 100% more active than the natural tachykinins kassinin (KASS) and eledoisin (ELED). Eledoisin was shown to be 50% less active than KASS, which is typical behaviour for an almost exclusively NK2 receptor population. Moreover, NK1- preferential peptides, namely substance P (SP) and physalaemin (PHYS), showed a lack of activity even when applied at high concentrations. 4. The results reported in this study show that lamb and sheep detrusor represent a good alternative model for the characterization of NK2-selective tachykinins.
Authors:
P Tucci; P Bolle; C Severini
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of autonomic pharmacology     Volume:  21     ISSN:  0144-1795     ISO Abbreviation:  J Auton Pharmacol     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-11-06     Completed Date:  2002-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8106455     Medline TA:  J Auton Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Affiliation:
Dipartimento di Farmacologia delle Sostanze Naturali e Fisiologia Generale, University of Rome 'La Sapienza', Ple Aldo Moro 5, 00185 Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Eledoisin / pharmacology
Female
Guinea Pigs
Kassinin / pharmacology
Male
Muscle, Smooth / drug effects*
Neurokinin A / pharmacology
Peptides, Cyclic / pharmacology
Receptors, Neurokinin-2 / drug effects
Sheep
Substance P / pharmacology
Tachykinins / metabolism*
Urinary Tract / drug effects*
Chemical
Reg. No./Substance:
0/Peptides, Cyclic; 0/Receptors, Neurokinin-2; 0/Tachykinins; 157351-81-0/MEN 10627; 33507-63-0/Substance P; 63968-82-1/Kassinin; 69-25-0/Eledoisin; 86933-74-6/Neurokinin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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