Document Detail

Effects of nanopatterned RGD peptide layer on electrochemical detection of neural cell chip.
MedLine Citation:
PMID:  20709522     Owner:  NLM     Status:  In-Process    
The cell-based chip is becoming a popular tool for monitoring living cell viability under various conditions. In this study, several biomaterials, such as synthetic Cys-(Arg-Gly-Asp)(4) (C(RGD)(4)), Arg-Gly-Asp-Multi Armed-Cys (RGD-MAP-C) peptide, and poly-L-lysine (PLL) nano-dots were fabricated on the gold surface of a neural cell chip. The material-dependent effects both on electrochemical signal detection in neural cells and on cellular adhesion were analyzed. The nano-dot structures were fabricated through a nanoporous alumina mask, and the structural formations were confirmed by scanning electron microscopy (SEM). PC12 cells were allowed to attach on several peptide nanopatterned surfaces, and electrochemical tools were applied to neural cells attached on the chip surface. The RGD-MAP-C peptide nanopatterned surface provided the strongest voltammetric signals when the cell was exposed to cyclic voltammetry (CV) and differential pulse voltammetry (DPV) after 48 h of incubation, which may largely be due to an enhanced affinity between cells and the Au surface. Chemical toxicity assessments were conducted in the fabricated cell chip, and they showed negative correlations between neural cell viability and the concentration of chemicals. In conclusion, a nanopatterned RGD-MAP-C layer improved cell-binding affinity to Au substrates and showed sufficient sensitivity for electrochemical detection of cell viability.
Md Abdul Kafi; Tae-Hyung Kim; Cheol-Heon Yea; Hyuncheol Kim; Jeong-Woo Choi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-21
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  26     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1359-65     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-Dong Mapo-Gu, Seoul 121-742, Republic of Korea.
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