| Effects of a nanoparticulate silica substrate on cell attachment of Candida albicans. | |
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MedLine Citation:
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PMID: 17309625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To investigate the influence of silica nanoparticles on the attachment and growth of Candida albicans cells. METHODS AND RESULTS: Spherical silica nanoparticles with diameters of 4, 7, 14 or 21 nm were attached to tissue culture polystyrene by a polycationic binding layer using a simple deposition procedure. The modified surfaces were shown to reduce the attachment and growth of C. albicans cells by a range of different measurements including microscopy, staining cells and measuring the amount of dye taken up and total cell activity measured using a dye reduction assay. For those cells that did attach and grow, the nanoparticle-coated surface inhibited the yeast to hyphal transition that is induced in the presence of serum. The greatest effect was observed for 7 and 14 nm diameter silica particles and we propose that the mechanism for these effects are related to either the topography of the surface or the slow dissolution of the bound silica. CONCLUSIONS: The attachment and growth of C. albicans is reduced by surface modification with silica nanoparticles. SIGNIFICANCE AND IMPACT OF THE STUDY: The modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of Candida sp. cells on medical devices thus, limiting the incidence of this pathogenic yeast in clinical environments. |
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Authors:
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B G Cousins; H E Allison; P J Doherty; C Edwards; M J Garvey; D S Martin; R L Williams |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 102 ISSN: 1364-5072 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-20 Completed Date: 2007-08-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 757-65 Citation Subset: IM |
Affiliation:
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School of Clinical Sciences, Division of Clinical Engineering, University of Liverpool, Liverpool, UK. kj26@liv.ac.uk <kj26@liv.ac.uk> |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Candida albicans
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growth & development,
physiology* Cell Adhesion / physiology Culture Media Indicators and Reagents / chemistry Microscopy, Atomic Force / methods Nanoparticles* Particle Size Polystyrenes Silicon Dioxide* Tetrazolium Salts / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Indicators and Reagents; 0/Polystyrenes; 0/Tetrazolium Salts; 117038-70-7/2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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