| Effects of mutant Ran/TC4 proteins on cell cycle progression. | |
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MedLine Citation:
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PMID: 8196659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ran/TC4, a member of the RAS gene superfamily, encodes an abundant nuclear protein that binds and hydrolyzes GTP. Transient expression of a Ran/TC4 mutant protein deficient in GTP hydrolysis blocked DNA replication, suggesting a role for Ran/TC4 in the regulation of cell cycle progression. To test this possibility, we exploited an efficient transfection system, involving the introduction of cDNAs in the pMT2 vector into 293/Tag cells, to analyze phenotypes associated with mutant and wild-type Ran/TC4 expression. Expression of a Ran/TC4 mutant protein deficient in GTP hydrolysis inhibited proliferation of transfected cells by arresting them predominantly in the G2, but also in the G1, phase of the cell cycle. Deletion of an acidic carboxy-terminal hexapeptide from the Ran/TC4 mutant did not alter its nuclear localization but did block its inhibitory effect on cell cycle progression. These data suggest that normal progression of the cell cycle is coupled to the operation of a Ran/TC4 GTPase cycle. Mediators of this coupling are likely to include the nuclear regulator of chromosome condensation 1 protein and the mitosis-promoting factor complex. |
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Authors:
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M Ren; E Coutavas; P D'Eustachio; M G Rush |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 14 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1994 Jun |
Date Detail:
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Created Date: 1994-06-24 Completed Date: 1994-06-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4216-24 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, NYU Medical Center, New York 10016. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M31469 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Cell Cycle / physiology* Cell Division / physiology Cell Line GTP Phosphohydrolases / metabolism GTP-Binding Proteins / metabolism* Genes, ras* Humans Kidney Kinetics Models, Biological Molecular Sequence Data Multigene Family* Nuclear Proteins / biosynthesis, genetics, metabolism* Oligodeoxyribonucleotides Sequence Deletion Sequence Homology, Amino Acid Transfection ran GTP-Binding Protein |
| Grant Support | |
ID/Acronym/Agency:
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GM07827/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nuclear Proteins; 0/Oligodeoxyribonucleotides; EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.5.2/ran GTP-Binding Protein |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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