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Effects of a murine germ cell-specific knockout of Connexin 43 on Connexin expression in testis and fertility.
MedLine Citation:
PMID:  23188169     Owner:  NLM     Status:  Publisher    
Connexin 43 (Cx 43)-expressed by germ cells (GC), Sertoli cells (SC) and Leydig cells-is one of at least eleven Cx in the murine testis. A general knockout (KO) of Cx 43 in mice results in perinatal death and a SC-specific KO of Cx 43 (SCCx43KO) causes infertility of male mice by preventing the initiation of spermatogenesis. To further elucidate the role of Cx 43 in the testis, a new mouse model with a GC-specific KO of Cx 43 (GCCx43KO) was created by using the Cre/loxP recombination system. A transgenic mouse line expressing the Cre gene under the tissue non-specific alkaline phosphatase promoter and a transgenic floxed Cx 43-LacZ mouse line were mated. The resulting F1-generation was backcrossed with homozygous Cx 43 floxed mice, and offspring was genotyped. Immunohistochemical analysis of testes of different aged homozygous mice revealed normal spermatogenesis and reduced Cx 43 immunoreactions. RT-qPCR and Western blots showed a downregulation of Cx 43 mRNA and protein, and a nearly unchanged mRNA expression of Cx 26, Cx 33 and Cx 45 in pubertal and adult KO mice. Western blots revealed considerable immunoreactive bands for Cx 26 and Cx 45. Male and female homozygous GCCx43KO mice were viable and fertile. Our data suggest, in contrast to inter SC and inter SC-GC cross talk in SCCx43KO mice which depends selectively on Cx 43 expression, that Cx 43 in GC seems not to be essential in GC-SC communication, when other Cx persist to be expressed.
Sabine Günther; Daniela Fietz; Karola Weider; Martin Bergmann; Ralph Brehm
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  Transgenic research     Volume:  -     ISSN:  1573-9368     ISO Abbreviation:  Transgenic Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9209120     Medline TA:  Transgenic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University Giessen, Frankfurter Str. 98, 35392, Giessen, Germany.
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